Developmental Toxicity Assessment Using Zebrafish-Based High-Throughput Screening

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2023 Sep 5

PMID 37668905

Authors

Subham Dasgupta

Michael T Simonich

Robyn L Tanguay

Affiliations

Soon will be listed here.

Abstract

Zebrafish-based high-throughput screening has been extensively used to study toxicological profiles of individual chemicals and mixtures, identify novel toxicants, and study modes of action to prioritize chemicals for further testing and policy decisions. Within this chapter, we describe a protocol for automated zebrafish developmental high-throughput screening in our laboratory, with emphasis on exposure setups, morphological and behavioral readouts, and quality control.

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PMID: 39459322 PMC: 11510690. DOI: 10.3390/molecules29204954.

References

Truong L, Tanguay R . Evaluation of Embryotoxicity Using the Zebrafish Model. Methods Mol Biol. 2017; 1641:325-333. DOI: 10.1007/978-1-4939-7172-5_18. View

Garcia G, Noyes P, Tanguay R . Advancements in zebrafish applications for 21st century toxicology. Pharmacol Ther. 2016; 161:11-21. PMC: 4851906. DOI: 10.1016/j.pharmthera.2016.03.009. View

Mandrell D, Truong L, Jephson C, Sarker M, Moore A, Lang C . Automated zebrafish chorion removal and single embryo placement: optimizing throughput of zebrafish developmental toxicity screens. J Lab Autom. 2012; 17(1):66-74. PMC: 3327291. DOI: 10.1177/2211068211432197. View

Barton C, Johnson E, Tanguay R . Facility Design and Health Management Program at the Sinnhuber Aquatic Research Laboratory. Zebrafish. 2016; 13 Suppl 1:S39-43. PMC: 4931725. DOI: 10.1089/zeb.2015.1232. View

Kimmel C, Ballard W, Kimmel S, Ullmann B, Schilling T . Stages of embryonic development of the zebrafish. Dev Dyn. 1995; 203(3):253-310. DOI: 10.1002/aja.1002030302. View