Carbapenem-resistant Harboring and : a Study on Their Transferability and Potential Dissemination Via Generating a Transferrable Hybrid Plasmid Mediated by IS

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2023 Sep 4

PMID 37664119

Authors

Feilong Zhang

Ziyao Li

Xinmeng Liu

Yanning Hu

Jiankang Zhao

Yulin Zhang

Yanyan Fan

Zichen Lei

Xinrui Yang

Zhihua Li

Chen Li

Yongli Wu

Binghuai Lu

Affiliations

Soon will be listed here.

Abstract

Introduction: The increase in clinical with dual carbapenemase has become a serious healthcare concern. It is essential to characterize the transferability and potential dissemination of - and -coharboring carbapenem-resistant (CRCF).

Methods: Four - and -coharboring CRCF strains were collected from our surveillance of the prevalence of carbapenem-resistant . The isolates were assessed using species identification, antimicrobial susceptibility testing, conjugation assays, whole-genome sequencing, plasmid stability, and fitness costs. Clonality, genome, plasmidome, and phylogeny were analyzed to reveal potential dissemination.

Results: Three ST523 - and -coharboring CRCF strains, collected from the same hospital within 1 month, exhibited high homology (both identity and coverage >99%), implying clonal dissemination and a small-scale outbreak. Moreover, the and genes were coharbored on an IncR plasmid, probably generated by a -harboring plasmid acquiring , in these three strains. Importantly, the IncR plasmid may form a transferable hybrid plasmid, mediated by IS via transposition, with another IncFII plasmid included in the same strain. Furthermore, the and of the fourth CRCF strain are located on two different non-transferable plasmids lacking complete transfer elements. Additionally, throughout the course of the 10-day continuous passage, the genetic surroundings of in four CRCF strains were gradually excised from their plasmids after the 8th day, whereas they maintained 100% retention for . Genome and plasmidome analyses revealed that - or -harboring were divergent, and these plasmids have high homology to plasmids of other .

Conclusion: Clonal dissemination of ST523 - and -coharboring CRCF strains was detected, and we first reported and concomitantly located on one plasmid, which could be transferred with mediation by IS via transposition. Continued surveillance should urgently be implemented.

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