Citrobacter Freundii Carrying BlaKPC-2 and BlaNDM-1: Characterization by Whole Genome Sequencing

Overview

Journal Sci Rep

Specialty Science

Date 2016 Jul 29

PMID 27465241

Citations 22

Authors

Wenjing Wu

Bjorn Espedido

Yu Feng

Zhiyong Zong

Affiliations

Soon will be listed here.

Abstract

A carbapenem-resistant Citrobacter freundii strain WCHCF65 was recovered from hospital sewage and was characterized by genome sequencing and conjugation experiments. The strain carried nine genes encoding β-lactamases including two carbapenemase genes, blaNDM-1 and blaKPC-2. blaNDM-1 was carried on an IncX3 plasmid, which was identical to a plasmid found in a local Escherichia coli, suggesting interspecies horizontal transfer. blaKPC-2 was bracketed by two copies of insertion sequence ISKpn19, which could form a composite transposon with the potential to mobilize blaKPC-2, on a new type of plasmid. The coexistence of blaNDM-1 and blaKPC-2 conferred higher levels of resistance to carbapenems compared with blaNDM-1 or blaKPC-2 alone. The coexistence of these carbapenemase genes, on two different plasmids, in one strain may allow new genetic platforms to be generated to mediate their spread.

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