Establishment of RNA Interference Genetic Transformation System and Functional Analysis of FlbA Gene in

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2023 Aug 26

PMID 37629189

Authors

Tian Gan

Huanli An

Ming Tang

Hui Chen

Affiliations

Soon will be listed here.

Abstract

is a pathogenic fungus of that is epidemic in the Qinling Mountains. However, an effective gene interference strategy is needed to characterize the pathogenic genes in this fungus on a functional level. Using the RNA silencing vector pSilent-1 as a template, we established an RNA interference genetic transformation system mediated by GV3101, which is suitable for the gene study for by homologous recombination and strain interference system screening. The LqFlbA gene was silenced using the RNA interference approach described above, and the resulting transformants displayed various levels of silencing with a gene silencing effectiveness ranging from 41.8% to 91.4%. The LqFlbA-RNAi mutant displayed altered colony morphology, sluggish mycelium growth, and diminished pathogenicity toward the host in comparison to the wild type. The results indicate that this method provides a useful reverse genetic system for studying the gene function of , and that plays a crucial role in the growth, development, and pathogenicity of .

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