Resequencing of Global Lotus Corniculatus Accessions Reveals Population Distribution and Genetic Loci, Associated with Cyanogenic Glycosides Accumulation and Growth Traits

Overview

Journal BMC Biol

Publisher Biomed Central

Specialty Biology

Date 2023 Aug 17

PMID 37592232

Authors

Cheng Chen

Kaixuan Zhang

Fu Liu

Xia Wang

Yang Yao

Xiaolei Niu

Yuqi He

Jun Hong

Fang Liu

Qiu Gao

Yi Zhang

Yurong Li

Meijuan Wang

Jizhen Lin

Yu Fan

Kui Ren

Lunhao Shen

Bin Gao

Xue Ren

Weifei Yang

Milen I Georgiev

Xinquan Zhang

Meiliang Zhou

Affiliations

Soon will be listed here.

Abstract

Background: Lotus corniculatus is a widely distributed perennial legume whose great adaptability to different environments and resistance to barrenness make it an excellent forage and ecological restoration plant. However, its molecular genetics and genomic relationships among populations are yet to be uncovered.

Result: Here we report on a genomic variation map from worldwide 272 L. corniculatus accessions by genome resequencing. Our analysis suggests that L. corniculatus accessions have high genetic diversity and could be further divided into three subgroups, with the genetic diversity centers were located in Transcaucasia. Several candidate genes and SNP site associated with CNglcs content and growth traits were identified by genome-wide associated study (GWAS). A non-synonymous in LjMTR was responsible for the decreased expression of CNglcs synthesis genes and LjZCD was verified to positively regulate CNglcs synthesis gene CYP79D3. The LjZCB and an SNP in LjZCA promoter were confirmed to be involved in plant growth.

Conclusion: This study provided a large number of genomic resources and described genetic relationship and population structure among different accessions. Moreover, we attempt to provide insights into the molecular studies and breeding of CNglcs and growth traits in L. corniculatus.

Citing Articles

De novo genome assembly of white clover (Trifolium repens L.) reveals the role of copy number variation in rapid environmental adaptation.

Kuo W, Wright S, Small L, Olsen K BMC Biol. 2024; 22(1):165.

PMID: 39113037 PMC: 11305067. DOI: 10.1186/s12915-024-01962-6.

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