Investigating Prenatal and Perinatal Factors on Meconium Microbiota: a Systematic Review and Cohort Study

Overview

Journal Pediatr Res

Specialties Biology
Pediatrics

Date 2023 Aug 17

PMID 37591927

Authors

Jenni Turunen

Mysore V Tejesvi

Niko Paalanne

Tytti Pokka

Sajeen Bahadur Amatya

Surbhi Mishra

Anna Kaisanlahti

Justus Reunanen

Terhi Tapiainen

Affiliations

Soon will be listed here.

Abstract

Background: The first-pass meconium has been suggested as a proxy for the fetal gut microbiota because it is formed in utero. This systematic review and cohort study investigated how pre- and perinatal factors influence the composition of the meconium microbiota.

Methods: We performed the systematic review using Covidence by searching PubMed, Scopus, and Web of Science databases with the search terms "meconium microbiome" and "meconium microbiota". In the cohort study, we performed 16 S rRNA gene sequencing on 393 meconium samples and analyzed the sequencing data using QIIME2.

Results: Our systematic review identified 69 studies exploring prenatal factors, immediate perinatal factors, and microbial composition in relation to subsequent health of infants but gave only limited comparative evidence regarding factors related to the composition of the meconium microbiota. The cohort study pointed to a low-biomass microbiota consisting of the phyla Firmicutes, Proteobacteria and Actinobacteriota and the genera Staphylococcus, Escherichia-Shigella and Lactobacillus, and indicated that immediate perinatal factors affected the composition of the meconium microbiota more than did prenatal factors.

Conclusions: This finding supports the idea that the meconium microbiota mostly starts developing during delivery.

Impact: It is unclear when the first-pass meconium microbiota develops, and what are the sources of the colonization. In this systematic review, we found 69 studies exploring prenatal factors, immediate perinatal factors, and microbial composition relative to subsequent health of infants, but there was no consensus on the factors affecting the meconium microbiota development. In this cohort study, immediate perinatal factors markedly affected the meconium microbiota development while prenatal factors had little effect on it. As the meconium microbiota composition was influenced by immediate perinatal factors, the present study supports the idea that the initial gut microbiota develops mainly during delivery.

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