A Nuclear Receptor HR96-related Gene Underlies Large Trans-driven Differences in Detoxification Gene Expression in a Generalist Herbivore

Overview

Journal Nat Commun

Specialty Biology

Date 2023 Aug 17

PMID 37591878

Authors

Meiyuan Ji

Marilou Vandenhole

Berdien De Beer

Sander De Rouck

Ernesto Villacis-Perez

Rene Feyereisen

Richard M Clark

Thomas Van Leeuwen

Affiliations

Soon will be listed here.

Abstract

The role, magnitude, and molecular nature of trans-driven expression variation underlying the upregulation of detoxification genes in pesticide resistant arthropod populations has remained enigmatic. In this study, we performed expression quantitative trait locus (eQTL) mapping (n = 458) between a pesticide resistant and a susceptible strain of the generalist herbivore and crop pest Tetranychus urticae. We found that a single trans eQTL hotspot controlled large differences in the expression of a subset of genes in different detoxification gene families, as well as other genes associated with host plant use. As established by additional genetic approaches including RNAi gene knockdown, a duplicated gene with a nuclear hormone receptor HR96-related ligand-binding domain was identified as causal for the expression differences between strains. The presence of a large family of HR96-related genes in T. urticae may enable modular control of detoxification and host plant use genes, facilitating this species' known and rapid evolution to diverse pesticides and host plants.

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