Cooperative Regulation of C1-domain Membrane Recruitment Polarizes Atypical Protein Kinase C

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 2023 Aug 17

PMID 37589718

Authors

Kimberly A Jones

Michael L Drummond

Rhiannon R Penkert

Kenneth E Prehoda

Affiliations

Soon will be listed here.

Abstract

Recruitment of the Par complex protein atypical protein kinase C (aPKC) to a specific membrane domain is a key step in the polarization of animal cells. While numerous proteins and phospholipids interact with aPKC, how these interactions cooperate to control its membrane recruitment has been unknown. Here, we identify aPKC's C1 domain as a phospholipid interaction module that targets aPKC to the membrane of Drosophila neural stem cells (NSCs). The isolated C1 binds the NSC membrane in an unpolarized manner during interphase and mitosis and is uniquely sufficient among aPKC domains for targeting. Other domains, including the catalytic module and those that bind the upstream regulators Par-6 and Bazooka, restrict C1's membrane targeting activity-spatially and temporally-to the apical NSC membrane during mitosis. Our results suggest that aPKC polarity results from cooperative activation of autoinhibited C1-mediated membrane binding activity.

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