LED Therapy Plus Idebenone Treatment Targeting Calcium and Mitochondrial Signaling Pathways in Dystrophic Muscle Cells

Overview

Journal Cell Stress Chaperones

Publisher Elsevier

Specialty Cell Biology

Date 2023 Aug 14

PMID 37578579

Authors

Heloina Nathallie Mariano da Silva

Daniela Sayuri Mizobuti

Valeria Andrade Pereira

Guilherme Luiz da Rocha

Marcos Vinicius da Cruz

Andre Gustavo de Oliveira

Leonardo Reis Silveira

Elaine Minatel

Affiliations

Soon will be listed here.

Abstract

Intracellular calcium dysregulation, oxidative stress, and mitochondrial dysfunction are some of the main pathway contributors towards disease progression in Duchenne muscular dystrophy (DMD). This study is aimed at investigating the effects of light emitting diode therapy (LEDT) and idebenone antioxidant treatment, applied alone or together in dystrophic primary muscle cells from mdx mice, the experimental model of DMD. Mdx primary muscle cells were submitted to LEDT and idebenone treatment and evaluated for cytotoxic effects and calcium and mitochondrial signaling pathways. LEDT and idebenone treatment showed no cytotoxic effects on the dystrophic muscle cells. Regarding the calcium pathways, after LEDT and idebenone treatment, a significant reduction in intracellular calcium content, calpain-1, calsequestrin, and sarcolipin levels, was observed. In addition, a significant reduction in oxidative stress level markers, such as HO, and 4-HNE levels, was observed. Regarding mitochondrial signaling pathways, a significant increase in oxidative capacity (by OCR and OXPHOS levels) was observed. In addition, the PGC-1α, SIRT-1, and PPARδ levels were significantly higher in the LEDT plus idebenone treated-dystrophic muscle cells. Together, the findings suggest that LEDT and idebenone treatment, alone or in conjunction, can modulate the calcium and mitochondrial signaling pathways, such as SLN, SERCA 1, and PGC-1α, contributing towards the improvement of the dystrophic phenotype in mdx muscle cells. In addition, data from the LEDT plus idebenone treatment showed slightly better results than those of each separate treatment in terms of SLN, OXPHOS, and SIRT-1.

Citing Articles

LEDT and Idebenone treatment modulate autophagy and improve regenerative capacity in the dystrophic muscle through an AMPK-pathway.

da Silva H, Fernandes E, Pereira V, Mizobuti D, Covatti C, da Rocha G PLoS One. 2024; 19(3):e0300006.

PMID: 38498472 PMC: 10947673. DOI: 10.1371/journal.pone.0300006.

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