Limosilactobacillus Reuteri as Sustainable Biological Control Agent Against Toxigenic Fusarium Verticillioides

Overview

Journal Braz J Microbiol

Specialty Microbiology

Date 2023 Aug 2

PMID 37531006

Authors

Melissa Tiemi Hirozawa

Mario Augusto Ono

Igor Massahiro de Souza Suguiura

Sandra Garcia

Jaqueline Gozzi Bordini

Ismael Rodrigues Amador

Elisa Yoko Hirooka

Elisabete Yurie Sataque Ono

Affiliations

Soon will be listed here.

Abstract

Corn contamination with Fusarium verticillioides (Sacc.) Nirenberg is a worldwide problem that affects yield and grain quality resulting in severe economic losses and implications for food safety. Control of F. verticillioides is a challenge, but lactic acid bacteria (LAB) has high potential as a biological control agent. In this study, the antifungal effect of Limosilactobacillus reuteri (formerly Lactobacillus reuteri) LR-92 against F. verticillioides 97L was investigated. Cell-free supernatant (CFS) from L. reuteri showed concentration-dependent fungicidal and fungistatic activity against F. verticillioides 97L. The antifungal compounds from CFS showed heat stability and pH dependence, and antifungal activity was not affected by treatment with proteolytic enzymes. High-performance liquid chromatography analysis indicated that L. reuteri LR-92 produces lactic and acetic acids. After liquid-liquid extraction, electrospray ionization mass spectrometry analysis of the active ethyl acetate fraction containing antifungal compounds revealed the production of 3-phenyllactic acid, cyclo-(L-Pro-L-Leu), cyclo-(L-Pro-L-Phe), and cyclo-(L-Phe-trans-4-OH-L-Pro). L. reuteri LR-92 has potential as a biocontrol agent for F. verticillioides and contributes to food safety.

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