Acute and Repeated Dose 28-Day Oral Toxicity Study of the Aqueous Extracts from the Leafy Stem and Fruit of D.Royen EX.L in Wistar Rats

Overview

Journal J Toxicol

Publisher Wiley

Specialty Toxicology

Date 2023 Jul 31

PMID 37520119

Authors

Gerard Bessan Dossou-Agoin

Maxime Machioud Sangare-Oumar

Teniola Isabelle Sacramento

Mariette Sindete

Egnon Jacques Hougbenou-Houngla

Nounagnon Darius Tossavi

Simon Azonbakin

Adam Gbankoto

Affiliations

Soon will be listed here.

Abstract

Background: () is used in folk medicine for treatment of male infertility. However, scientific data on its safety are limited.

Objective: This study was carried out to assess the acute and repeated dose 28-day oral toxicity of the aqueous extracts from leafy stem and fruit in Wistar rats.

Methods: The acute toxicity test was performed according to the line 423 of the Organization for Economic Cooperation and Development (OECD) guidelines. The rats were randomly divided into three groups ( = 3). The control group received distilled water, while the experimental groups were given at a single dose, 5000 mg/kg of each extract. The repeated dose 28-day oral toxicity was performed according to the line 407 of the OECD guidelines. 35 rats divided into 7 groups of 5 male rats each were daily treated for 28 days with each extract at 200 mg/kg, 400 mg/kg, and 800 mg/kg, respectively. The in-life parameters were recorded during the follow-up. At the end of this study, organ weights, hematology, biochemistry, and histology parameters were analyzed.

Results: In the acute oral toxicity test, there was no morbidity or mortality related to the treatments. Both extracts belong therefore to category 5 of the globally harmonized system (GHS) of classification. In the repeated dose 28-day oral toxicity test, both extracts did not alter animal's behavior. However, both extract administration led to proteinuria and renal damages.

Conclusion: leafy stem and fruit aqueous extracts exhibited potential nephrotoxicity. Therefore, care should be taken when they are used over an extended period.

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