A Novel Hydrogel Containing 4-methylcatechol for Skin Regeneration: in Vitro and in Vivo Study

Overview

Journal Biomed Eng Lett

Specialty Biomedical Engineering

Date 2023 Jul 31

PMID 37519882

Authors

Jilla Majidi Ghatar

Arian Ehterami

Simin Nazarnezhad

Maryam Sadat Hassani

Nariman Rezaei Kolarijani

Solmaz Mahami

Majid Salehi

Affiliations

Soon will be listed here.

Abstract

Skin damages are usual physical injuries and different studies have been done to improve wound healing. Hydrogel due to its properties like a moist environment and cooling wound site is a good option for wound treatment. In this study, we evaluated the consequence of using alginate/chitosan hydrogel contained various dosages of 4-Methylcatechol (0, 0.1, 1% (W/W)) on wound healing. After hydrogel fabrication, different tests like SEM, swelling, release, weight loss, and hemo- and cytocompatibility were done to characterize fabricated hydrogels. Finally, the rat model was used to assess Alginate/Chitosan hydrogel's therapeutic function containing 0.1 and 1% of 4-Methylcatechol. The pore size of hydrogel was between 24.5 ± 9 and 62.1 ± 11.63 µm and about 90% of hydrogel was lost after 14 days in the weight loss test. Blood compatibility and MTT assay showed that hydrogels were nontoxic and improved cell proliferation. In vivo test showed that Alginate/Chitosan/0.1%4-Methylcatechol improved wound healing and the results were significantly better than the gauze-treated wound. Our results showed dose depending effect of 4-Methylcatechol on wound healing. This study shows the treatment effect of 4-Methylcatechol on wound healing and the possibility of using it for treating skin injuries.

Citing Articles

The Peculiar H-Bonding Network of 4-Methylcatechol: A Coupled Diffraction and In Silico Study.

Lopresti M, Palin L, Calegari G, Milanesio M Molecules. 2024; 29(10).

PMID: 38792035 PMC: 11124409. DOI: 10.3390/molecules29102173.

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