Exploring the Antimicrobial, Anticancer, and Apoptosis Inducing Ability of Biofabricated Silver Nanoparticles Using Flower Buds Against the Human Osteosarcoma (MG-63) Cell Line Via Flow Cytometry

Overview

Journal Bioengineering (Basel)

Date 2023 Jul 29

PMID 37508848

Authors

Kariyellappa Nagaraja Shashiraj

Anil Hugar

Raju Suresh Kumar

Muthuraj Rudrappa

Meghashyama Prabhakara Bhat

Abdulrahman I Almansour

Karthikeyan Perumal

Sreenivasa Nayaka

Affiliations

Soon will be listed here.

Abstract

Biosynthesized nano-composites, such as silver nanoparticles (AgNPs), can be engineered to function as smart nano-biomedicine platforms for the detection and management of diverse ailments, such as infectious diseases and cancer. This study determined the eco-friendly fabrication of silver nanoparticles using (L.) Pers. flower buds and their efficacy against antimicrobial and anticancer activities. The UV-Visible spectrum was found at 413 nm showing a typical resonance spectrum for flower bud extract-assisted silver nanoparticles (Ls-AgNPs). Fourier transform infrared analysis revealed the presence of amines, halides, and halogen compounds, which were involved in the reduction and capping agent of AgNP formation. X-ray diffraction analysis revealed the face-centered cubic crystals of NPs. Energy dispersive X-ray verified the weight of 39.80% of silver (Ag), TEM analysis revealed the particles were spherical with a 10.27 to 62.5 nm range, and dynamic light scattering recorded the average particle size around 58.5 nm. Zeta potential showed a significant value at -39.4 mV, and finally, thermo-gravimetric analysis reported higher thermal stability of Ls-AgNPs. Further, the obtained Ls-AgNPs displayed good antimicrobial activity against clinical pathogens. In addition, a dose-dependent decrease in the anticancer activity by MTT assay on the osteosarcoma (MG-63) cell line showed a decrease in the cell viability with increasing in the concentration of Ls-AgNPs with an IC value of 37.57 µg/mL. Subsequently, an apoptotic/necrosis study was conducted with the help of Annexin-V/PI assay, and the results indicated a significant rise in early and late apoptosis cell populations. Therefore, green synthesized Ls-AgNPs were found to have potent antimicrobial and anticancer properties making them fascinating choices for future bio-medical implementations.

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References

Mousa A, El-Sammad N, Abdel-Halim A, Anwar N, Khalil W, Nawwar M . (L.) Pers Leaf Extract Attenuates Lung Tumorigenesis via Alleviating Oxidative Stress, Inflammation and Apoptosis. Biomolecules. 2019; 9(12). PMC: 6995620. DOI: 10.3390/biom9120871. View

Iravani S, Korbekandi H, Mirmohammadi S, Zolfaghari B . Synthesis of silver nanoparticles: chemical, physical and biological methods. Res Pharm Sci. 2015; 9(6):385-406. PMC: 4326978. View

Niu G, Yousefi B, Qujeq D, Marjani A, Asadi J, Wang Z . Melatonin and doxorubicin co-delivered via a functionalized graphene-dendrimeric system enhances apoptosis of osteosarcoma cells. Mater Sci Eng C Mater Biol Appl. 2020; 119:111554. DOI: 10.1016/j.msec.2020.111554. View

Nagaraja S, Nayaka S, Kumar R . Phytochemical Analysis, GC-MS Profiling, and In Vitro Evaluation of Biological Applications of Different Solvent Extracts of Leonotis nepetifolia (L.) R.Br. Flower Buds. Appl Biochem Biotechnol. 2022; 195(2):1197-1215. DOI: 10.1007/s12010-022-04201-2. View

Moteriya P, Chanda S . Synthesis and characterization of silver nanoparticles using Caesalpinia pulcherrima flower extract and assessment of their in vitro antimicrobial, antioxidant, cytotoxic, and genotoxic activities. Artif Cells Nanomed Biotechnol. 2016; 45(8):1556-1567. DOI: 10.1080/21691401.2016.1261871. View

Rajesh Kumar T, Murthy J, Narayana Rao M, Bhargava Y . Evaluation of silver nanoparticles synthetic potential of Couroupita guianensis Aubl., flower buds extract and their synergistic antibacterial activity. 3 Biotech. 2017; 6(1):92. PMC: 4801843. DOI: 10.1007/s13205-016-0407-9. View

Alharbi N, Govindarajan M, Kadaikunnan S, Khaled J, N Almanaa T, Alyahya S . Nanosilver crystals capped with Bauhinia acuminata phytochemicals as new antimicrobials and mosquito larvicides. J Trace Elem Med Biol. 2018; 50:146-153. DOI: 10.1016/j.jtemb.2018.06.016. View

Nayak D, Ashe S, Ranjan Rauta P, Kumari M, Nayak B . Bark extract mediated green synthesis of silver nanoparticles: Evaluation of antimicrobial activity and antiproliferative response against osteosarcoma. Mater Sci Eng C Mater Biol Appl. 2015; 58:44-52. DOI: 10.1016/j.msec.2015.08.022. View

Stiller C, Craft A, Corazziari I . Survival of children with bone sarcoma in Europe since 1978: results from the EUROCARE study. Eur J Cancer. 2001; 37(6):760-6. DOI: 10.1016/s0959-8049(01)00004-1. View

10.

Wang D, Markus J, Wang C, Kim Y, Mathiyalagan R, Aceituno V . Green synthesis of gold and silver nanoparticles using aqueous extract of Cibotium barometz root. Artif Cells Nanomed Biotechnol. 2016; 45(8):1548-1555. DOI: 10.1080/21691401.2016.1260580. View

11.

Iram S, Khan S, Ansary A, Arshad M, Siddiqui S, Ahmad E . Biogenic terbium oxide nanoparticles as the vanguard against osteosarcoma. Spectrochim Acta A Mol Biomol Spectrosc. 2016; 168:123-131. DOI: 10.1016/j.saa.2016.05.053. View

12.

Riaz M, Mutreja V, Sareen S, Ahmad B, Faheem M, Zahid N . Exceptional antibacterial and cytotoxic potency of monodisperse greener AgNPs prepared under optimized pH and temperature. Sci Rep. 2021; 11(1):2866. PMC: 7858571. DOI: 10.1038/s41598-021-82555-z. View

13.

Patra J, Das G, Fraceto L, Campos E, Del Pilar Rodriguez-Torres M, Acosta-Torres L . Nano based drug delivery systems: recent developments and future prospects. J Nanobiotechnology. 2018; 16(1):71. PMC: 6145203. DOI: 10.1186/s12951-018-0392-8. View

14.

Nagaraja S, Niazi S, Bepari A, Assiri R, Nayaka S . Flower Bud Extract Mediated Green Synthesis of Silver Nanoparticles, Their Characterization, and Evaluation of Biological Applications. Materials (Basel). 2022; 15(24). PMC: 9781718. DOI: 10.3390/ma15248990. View

15.

De Giglio E, Cafagna D, Cometa S, Allegretta A, Pedico A, Giannossa L . An innovative, easily fabricated, silver nanoparticle-based titanium implant coating: development and analytical characterization. Anal Bioanal Chem. 2012; 405(2-3):805-16. DOI: 10.1007/s00216-012-6293-z. View

16.

Lo Vasco V, Leopizzi M, Di Maio V, Della Rocca C . U-73122 reduces the cell growth in cultured MG-63 ostesarcoma cell line involving Phosphoinositide-specific Phospholipases C. Springerplus. 2016; 5:156. PMC: 4766154. DOI: 10.1186/s40064-016-1768-6. View

17.

Lekshmi A, Varadarajan S, Lupitha S, Indira D, Mathew K, Chandrasekharan Nair A . A quantitative real-time approach for discriminating apoptosis and necrosis. Cell Death Discov. 2017; 3:16101. PMC: 5253725. DOI: 10.1038/cddiscovery.2016.101. View

18.

Lakhan M, Chen R, Shar A, Chand K, Shah A, Ahmed M . Eco-friendly green synthesis of clove buds extract functionalized silver nanoparticles and evaluation of antibacterial and antidiatom activity. J Microbiol Methods. 2020; 173:105934. DOI: 10.1016/j.mimet.2020.105934. View

19.

OBRIEN M, Bolton W . Comparison of cell viability probes compatible with fixation and permeabilization for combined surface and intracellular staining in flow cytometry. Cytometry. 1995; 19(3):243-55. DOI: 10.1002/cyto.990190308. View

20.

Muthukumaran U, Govindarajan M, Rajeswary M, Hoti S . Synthesis and characterization of silver nanoparticles using Gmelina asiatica leaf extract against filariasis, dengue, and malaria vector mosquitoes. Parasitol Res. 2015; 114(5):1817-27. DOI: 10.1007/s00436-015-4368-4. View