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Effect of Pressure Conditions in Uterine Decellularization Using Hydrostatic Pressure on Structural Protein Preservation

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Date 2023 Jul 29
PMID 37508841
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Abstract

Uterine regeneration using decellularization scaffolds provides a novel treatment for uterine factor infertility. Decellularized scaffolds require maximal removal of cellular components and minimal damage to the extracellular matrix (ECM). Among many decellularization methods, the hydrostatic pressure (HP) method stands out due to its low cytotoxicity and superior ECM preservation compared to the traditional detergent methods. Conventionally, 980 MPa was utilized in HP decellularization, including the first successful implementation of uterine decellularization previously reported by our team. However, structural protein denaturation caused by exceeding pressure led to a limited regeneration outcome in our previous research. This factor urged the study on the effects of pressure conditions in HP methods on decellularized scaffolds. The authors, therefore, fabricated a decellularized uterine scaffold at varying pressure conditions and evaluated the scaffold qualities from the perspective of cell removal and ECM preservation. The results show that by using lower decellularization pressure conditions of 250 MPa, uterine tissue can be decellularized with more preserved structural protein and mechanical properties, which is considered to be promising for decellularized uterine scaffold fabrication applications.

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References
1.
Zemmyo D, Yamamoto M, Miyata S . Fundamental Study of Decellularization Method Using Cyclic Application of High Hydrostatic Pressure. Micromachines (Basel). 2020; 11(11). PMC: 7696941. DOI: 10.3390/mi11111008. View

2.
Cui W . Mother or nothing: the agony of infertility. Bull World Health Organ. 2010; 88(12):881-2. PMC: 2995184. DOI: 10.2471/BLT.10.011210. View

3.
Daryabari S, Kajbafzadeh A, Fendereski K, Ghorbani F, Dehnavi M, Rostami M . Development of an efficient perfusion-based protocol for whole-organ decellularization of the ovine uterus as a human-sized model and in vivo application of the bioscaffolds. J Assist Reprod Genet. 2019; 36(6):1211-1223. PMC: 6603122. DOI: 10.1007/s10815-019-01463-4. View

4.
Hellstrom M, Moreno-Moya J, Bandstein S, Bom E, Akouri R, Miyazaki K . Bioengineered uterine tissue supports pregnancy in a rat model. Fertil Steril. 2016; 106(2):487-496.e1. DOI: 10.1016/j.fertnstert.2016.03.048. View

5.
Miyazaki K, Maruyama T . Partial regeneration and reconstruction of the rat uterus through recellularization of a decellularized uterine matrix. Biomaterials. 2014; 35(31):8791-8800. DOI: 10.1016/j.biomaterials.2014.06.052. View