Cobalt-Nitrogen Co-Doped Carbon As Highly Efficient Oxidase Mimics for Colorimetric Assay of Nitrite

Overview

Journal Biosensors (Basel)

Specialty Biotechnology

Date 2023 Jul 28

PMID 37504147

Authors

Dalei Lin

Shuzhi Wu

Shushu Chu

Yizhong Lu

Affiliations

Soon will be listed here.

Abstract

Transition metal-N-doped carbon has been demonstrated to mimic natural enzyme activity; in this study, cobalt-nitrogen co-doped carbon (Co-N-C) nanomaterial was developed, and it could be an oxidase mimic. Firstly, Co-N-C with oxidase-like activity boosts the chromogenic reaction of 3,3',5,5'-tetramethylbenzidine (TMB) to produce the oxidized TMB (oxTMB). And the aromatic primary amino group of oxTMB reacts with nitrite (NO) to form diazo groups. Based on this background, we developed a cascade system of a Co-N-C-catalyzed oxidation reaction and a diazotization reaction for nitrite determination. The low detection limit (0.039 μM) indicates that Co-N-C is superior compared with the vast majority of previously reported nitrite assays. This study not only provides a novel nanozyme with sufficiently dispersed active sites, but it also further applies it to the determination of nitrite, which is expected to expand the application of nanozymes in colorimetric analysis.

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