Identifying, Characterizing, and Engineering a Phenolic Acid-Responsive Transcriptional Factor from

Overview

Journal ACS Synth Biol

Publisher American Chemical Society

Specialties Biotechnology
Molecular Biology

Date 2023 Jul 27

PMID 37499217

Authors

Chenyi Li

Yuyang Zhou

Yusong Zou

Tian Jiang

Xinyu Gong

Yajun Yan

Affiliations

Soon will be listed here.

Abstract

Transcriptional factors-based biosensors are commonly used in metabolic engineering for inducible control of gene expression and related applications such as high-throughput screening and dynamic pathway regulations. Mining for novel transcriptional factors is essential for expanding the usability of these toolsets. Here, we report the identification, characterization, and engineering of a phenolic acid responsive regulator PadR from (BaPadR). This BaPadR-based biosensor system showed a unique ligand preference and exhibited a high output strength comparable to that of commonly used inducible expression systems. Through engineering the DNA binding region of BaPadR, we further enhanced the dynamic range of the biosensor system. The DNA sequences that are responsible for BaPadR recognition were located by promoter truncation and hybrid promoter building. To further explore the tunability of the sensor system, base substitutions were performed on the BaPadR binding region of the phenolic acid decarboxylase promoter (P) and the hybrid promoter. This novel biosensor system can serve as a valuable tool in future synthetic biology applications.

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