Human Neutrophils Communicate Remotely Via Calcium-dependent Glutamate-induced Glutamate Release

Overview

Journal iScience

Publisher Cell Press

Date 2023 Jul 27

PMID 37496680

Authors

Olga Kopach

Sergyi Sylantyev

Lucie Bard

Piotr Michaluk

Janosch P Heller

Ana Gutierrez Del Arroyo

Gareth L Ackland

Alexander V Gourine

Dmitri A Rusakov

Affiliations

Soon will be listed here.

Abstract

Neutrophils are white blood cells that are critical to acute inflammatory and adaptive immune responses. Their swarming-pattern behavior is controlled by multiple cellular cascades involving calcium-dependent release of various signaling molecules. Previous studies have reported that neutrophils express glutamate receptors and can release glutamate but evidence of direct neutrophil-neutrophil communication has been elusive. Here, we hold semi-suspended cultured human neutrophils in patch-clamp whole-cell mode to find that calcium mobilization induced by stimulating one neutrophil can trigger an N-methyl-D-aspartate (NMDA) receptor-driven membrane current and calcium signal in neighboring neutrophils. We employ an enzymatic-based imaging assay to image, in real time, glutamate release from neutrophils induced by glutamate released from their neighbors. These observations provide direct evidence for a positive-feedback inter-neutrophil communication that could contribute to mechanisms regulating communal neutrophil behavior.

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