Ulvan/Silver Nanoparticle Hydrogel Films for Burn Wound Dressing

Overview

Journal Heliyon

Specialty Social Sciences

Date 2023 Jul 24

PMID 37483826

Authors

Evi Sulastri

Ronny Lesmana

Muhammad Sulaiman Zubair

Ahmed Fouad Abdelwahab Mohammed

Khaled M Elamin

Nasrul Wathoni

Affiliations

Soon will be listed here.

Abstract

Ulvan is a polysaccharide from green algae that shows good hydrogel film dressing characteristics. Silver nanoparticles (AgNP) can be incorporated into the hydrogel film to improve antibacterial properties and provide a potential burn treatment. In this study, we developed a novel hydrogel film wound dressing composed of ulvan and silver nanoparticles. Two concentrations (0.5 mM and 1 mM) of silver nitrate were used to produce ulvan-silver nanoparticles hydrogel film (UHF-AgNP0.5 and UHF-AgNP1), respectively. The physicochemical characteristics of the hydrogel films were evaluated, including particle size, zeta potential, Fourier transform infrared (FTIR), X-ray diffractometry (XRD), scanning electron microscope and energy-dispersive X-ray (SEM-EDX). Furthermore, the in vitro antimicrobial activity, and second-degree burn wound healing test were evaluated. The UHF-AgNP0.5 showed the highest antimicrobial activity compared to UHF-AgNP1 and UHF film. Meanwhile, an in vivo study using Wistar rats induced second-degree burns showed that UHF-AgNP0.5 significantly accelerated the healing process by regulating the inflammatory process, increasing re-epithelialization, and improving the vascularization process. Ulvan-silver nanoparticle hydrogel films have the ability to accelerate the healing of second-degree burns and are potential candidates for wound dressings.

Citing Articles

Advancing burn wound treatment: exploring hydrogel as a transdermal drug delivery system.

Goh M, Du M, Peng W, Saw P, Chen Z Drug Deliv. 2024; 31(1):2300945.

PMID: 38366562 PMC: 10878343. DOI: 10.1080/10717544.2023.2300945.

Formulation and Comprehensive Evaluation of Biohybrid Hydrogel Membranes Containing Doxycycline or Silver Nanoparticles.

Stan D, Ruta L, Bocancia-Mateescu L, Mirica A, Stan D, Micutz M Pharmaceutics. 2023; 15(12).

PMID: 38140037 PMC: 10747233. DOI: 10.3390/pharmaceutics15122696.

Thermal, morphology and bacterial analysis of pH-responsive sodium carboxyl methylcellulose/ fumaric acid/ acrylamide nanocomposite hydrogels: Synthesis and characterization.

Sudarsan S, Trofimov E, Franklin D, Venthan S, Guhanathan S, Rangappa S Heliyon. 2023; 9(11):e20939.

PMID: 37954319 PMC: 10637903. DOI: 10.1016/j.heliyon.2023.e20939.

References

Concordio-Reis P, Pereira C, Batista M, Sevrin C, Grandfils C, Marques A . Silver nanocomposites based on the bacterial fucose-rich polysaccharide secreted by Enterobacter A47 for wound dressing applications: Synthesis, characterization and in vitro bioactivity. Int J Biol Macromol. 2020; 163:959-969. DOI: 10.1016/j.ijbiomac.2020.07.072. View

Haider Naqvi S, Kiran U, Ali M, Jamal A, Hameed A, Ahmed S . Combined efficacy of biologically synthesized silver nanoparticles and different antibiotics against multidrug-resistant bacteria. Int J Nanomedicine. 2013; 8:3187-95. PMC: 3754765. DOI: 10.2147/IJN.S49284. View

Said M, Rehan M, El-Sheikh S, Zahran M, Abdel-Aziz M, Bechelany M . Multifunctional Hydroxyapatite/Silver Nanoparticles/Cotton Gauze for Antimicrobial and Biomedical Applications. Nanomaterials (Basel). 2021; 11(2). PMC: 7915402. DOI: 10.3390/nano11020429. View

Chen H, Lan G, Ran L, Xiao Y, Yu K, Lu B . A novel wound dressing based on a Konjac glucomannan/silver nanoparticle composite sponge effectively kills bacteria and accelerates wound healing. Carbohydr Polym. 2018; 183:70-80. DOI: 10.1016/j.carbpol.2017.11.029. View

Alahmad A, Al-Zereini W, Hijazin T, Al-Madanat O, Alghoraibi I, Al-Qaralleh O . Green Synthesis of Silver Nanoparticles Using L. Aqueous Extract with the Evaluation of Its Antibacterial Activity against Clinical and Food Pathogens. Pharmaceutics. 2022; 14(5). PMC: 9144328. DOI: 10.3390/pharmaceutics14051104. View

Mehwish H, Liu G, Rajoka M, Cai H, Zhong J, Song X . Therapeutic potential of Moringa oleifera seed polysaccharide embedded silver nanoparticles in wound healing. Int J Biol Macromol. 2021; 184:144-158. DOI: 10.1016/j.ijbiomac.2021.05.202. View

Cavassin E, de Figueiredo L, Otoch J, Seckler M, de Oliveira R, Fantinelli Franco F . Comparison of methods to detect the in vitro activity of silver nanoparticles (AgNP) against multidrug resistant bacteria. J Nanobiotechnology. 2015; 13:64. PMC: 4593215. DOI: 10.1186/s12951-015-0120-6. View

Burdusel A, Gherasim O, Grumezescu A, Mogoanta L, Ficai A, Andronescu E . Biomedical Applications of Silver Nanoparticles: An Up-to-Date Overview. Nanomaterials (Basel). 2018; 8(9). PMC: 6163202. DOI: 10.3390/nano8090681. View

Choi O, Hu Z . Size dependent and reactive oxygen species related nanosilver toxicity to nitrifying bacteria. Environ Sci Technol. 2008; 42(12):4583-8. DOI: 10.1021/es703238h. View

10.

Xu J, Li Y, Wang H, Zhu M, Feng W, Liang G . Enhanced Antibacterial and Anti-Biofilm Activities of Antimicrobial Peptides Modified Silver Nanoparticles. Int J Nanomedicine. 2021; 16:4831-4846. PMC: 8291838. DOI: 10.2147/IJN.S315839. View

11.

Tian H, Yin X, Zeng Q, Zhu L, Chen J . Isolation, structure, and surfactant properties of polysaccharides from Ulva lactuca L. from South China Sea. Int J Biol Macromol. 2015; 79:577-82. DOI: 10.1016/j.ijbiomac.2015.05.031. View

12.

Singla R, Soni S, Kulurkar P, Kumari A, S M, Patial V . In situ functionalized nanobiocomposites dressings of bamboo cellulose nanocrystals and silver nanoparticles for accelerated wound healing. Carbohydr Polym. 2016; 155:152-162. DOI: 10.1016/j.carbpol.2016.08.065. View

13.

Pansara C, Mishra R, Mehta T, Parikh A, Garg S . Formulation of Chitosan Stabilized Silver Nanoparticle-Containing Wound Healing Film: In Vitro and In Vivo Characterization. J Pharm Sci. 2020; 109(7):2196-2205. DOI: 10.1016/j.xphs.2020.03.028. View

14.

Ibrahim M, Amer M, Ibrahim H, Zaghloul E . Considerable Production of Ulvan from Ulva lactuca with Special Emphasis on Its Antimicrobial and Anti-fouling Properties. Appl Biochem Biotechnol. 2022; 194(7):3097-3118. PMC: 9205838. DOI: 10.1007/s12010-022-03867-y. View

15.

Massironi A, Morelli A, Grassi L, Puppi D, Braccini S, Maisetta G . Ulvan as novel reducing and stabilizing agent from renewable algal biomass: Application to green synthesis of silver nanoparticles. Carbohydr Polym. 2018; 203:310-321. DOI: 10.1016/j.carbpol.2018.09.066. View

16.

Iram F, Yasmeen A, Massey S, Iqbal M, Asim S, Irshad M . Synthesis of gold and silver nanoparticles by use of arabinoglucan from Lallemantia royleana. Int J Biol Macromol. 2021; 191:1137-1150. DOI: 10.1016/j.ijbiomac.2021.09.096. View

17.

Vinaik R, Barayan D, Shahrokhi S, Jeschke M . Management and prevention of drug resistant infections in burn patients. Expert Rev Anti Infect Ther. 2019; 17(8):607-619. PMC: 6996139. DOI: 10.1080/14787210.2019.1648208. View

18.

Yu S, Yin Y, Liu J . Silver nanoparticles in the environment. Environ Sci Process Impacts. 2014; 15(1):78-92. DOI: 10.1039/c2em30595j. View

19.

Martinez-Higuera A, Rodriguez-Beas C, Villalobos-Noriega J, Arizmendi-Grijalva A, Ochoa-Sanchez C, Larios-Rodriguez E . Hydrogel with silver nanoparticles synthesized by Mimosa tenuiflora for second-degree burns treatment. Sci Rep. 2021; 11(1):11312. PMC: 8163746. DOI: 10.1038/s41598-021-90763-w. View

20.

Zia T, Usman M, Sabir A, Shafiq M, Khan R . Development of inter-polymeric complex of anionic polysaccharides, alginate/k-carrageenan bio-platform for burn dressing. Int J Biol Macromol. 2020; 157:83-95. DOI: 10.1016/j.ijbiomac.2020.04.157. View