Lycopene Enhances Bone Neoformation in Calvaria Bone Defects of Ovariectomized Rats

Overview

Journal Braz Dent J

Specialty Dentistry

Date 2023 Jul 19

PMID 37466525

Authors

Vitoria Ricardo

Luiz Gustavo de Sousa

Isabela Hallak Regalo

Dimitrius Leonardo Pitol

Karina Fittipaldi Bombonato-Prado

Simone Cecilio Hallak Regalo

Selma Siessere

Affiliations

Soon will be listed here.

Abstract

Osteoporosis can affect a significant part of the population and fractures are the most common complications associated with this disease, leading to high public health costs. Thus, the prevention of fractures is relevant to individuals with signs and symptoms as well as to the health system. Postmenopausal osteoporosis has been associated with oxidative stress, emphasizing the importance of an efficient defense system to maintain bone health. Lycopene is a carotenoid with antioxidant properties that may stimulate osteoblastogenesis and inhibit osteoclastogenesis. The purpose of this investigation was to analyze the influence of lycopene in the bone neoformation of calvaria defects in ovariectomized rats utilizing the concentration of 45 mg/kg. Wistar Hannover female rats were divided into ovariectomized and sham groups. The ovariectomized animals received 45 mg/kg lycopene (OvxL) or water (Ovx) by daily gavage the day after ovariectomy/sham surgery for 16 weeks. Twelve weeks after ovariectomy, there were performed 5-mm calvaria defects followed by euthanasia after 4 weeks. Samples of bone tissue were collected to perform morphological and morphometrical analysis of the neoformed bone area, and percentage with Software Image J. Morphological evaluation showed mature bone with more osteocytes in the group OVxL when compared to the other groups. The morphometrical analysis demonstrated a significant increase of bone neoformation in the group OvxL (p<0.05). The data obtained suggest that lycopene benefits bone repair in the absence of estrogenic hormones.

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References

Camara C, Zhou L, Ma Y, Zhu L, Yu D, Zhao Y . Effect of ovariectomy on serum adiponectin levels and visceral fat in rats. J Huazhong Univ Sci Technolog Med Sci. 2014; 34(6):825-829. DOI: 10.1007/s11596-014-1360-7. View

Russo C, Ferro Y, Maurotti S, Salvati M, Mazza E, Pujia R . Lycopene and bone: an in vitro investigation and a pilot prospective clinical study. J Transl Med. 2020; 18(1):43. PMC: 6990577. DOI: 10.1186/s12967-020-02238-7. View

Chen C, Wang L, Tulu U, Arioka M, Moghim M, Salmon B . An osteopenic/osteoporotic phenotype delays alveolar bone repair. Bone. 2018; 112:212-219. DOI: 10.1016/j.bone.2018.04.019. View

Castro M, Duarte N, Nascimento P, Magno M, Fagundes N, Flores-Mir C . Antioxidants as Adjuvants in Periodontitis Treatment: A Systematic Review and Meta-Analysis. Oxid Med Cell Longev. 2019; 2019:9187978. PMC: 6679881. DOI: 10.1155/2019/9187978. View

Scalize P, Bombonato-Prado K, Sousa L, Rosa A, Beloti M, Semprini M . Poly(Vinylidene Fluoride-Trifluorethylene)/barium titanate membrane promotes de novo bone formation and may modulate gene expression in osteoporotic rat model. J Mater Sci Mater Med. 2016; 27(12):180. DOI: 10.1007/s10856-016-5799-x. View

Zhou Q, Zhu L, Zhang D, Li N, Li Q, Dai P . Oxidative Stress-Related Biomarkers in Postmenopausal Osteoporosis: A Systematic Review and Meta-Analyses. Dis Markers. 2016; 2016:7067984. PMC: 4995322. DOI: 10.1155/2016/7067984. View

Oliveira G, Vargas-Sanchez P, Fernandes R, Ricoldi M, Semeghini M, Pitol D . Lycopene influences osteoblast functional activity and prevents femur bone loss in female rats submitted to an experimental model of osteoporosis. J Bone Miner Metab. 2018; 37(4):658-667. DOI: 10.1007/s00774-018-0970-8. View

Rashki Kemmak A, Rezapour A, Jahangiri R, Nikjoo S, Farabi H, Soleimanpour S . Economic burden of osteoporosis in the world: A systematic review. Med J Islam Repub Iran. 2021; 34:154. PMC: 7787041. DOI: 10.34171/mjiri.34.154. View

Ezzat-Zadeh Z, Kim J, Bryant Chase P, Arjmandi B . The Cooccurrence of Obesity, Osteoporosis, and Sarcopenia in the Ovariectomized Rat: A Study for Modeling Osteosarcopenic Obesity in Rodents. J Aging Res. 2017; 2017:1454103. PMC: 5471594. DOI: 10.1155/2017/1454103. View

10.

Domazetovic V, Marcucci G, Iantomasi T, Brandi M, Vincenzini M . Oxidative stress in bone remodeling: role of antioxidants. Clin Cases Miner Bone Metab. 2017; 14(2):209-216. PMC: 5726212. DOI: 10.11138/ccmbm/2017.14.1.209. View

11.

Iimura Y, Agata U, Takeda S, Kobayashi Y, Yoshida S, Ezawa I . The protective effect of lycopene intake on bone loss in ovariectomized rats. J Bone Miner Metab. 2014; 33(3):270-8. DOI: 10.1007/s00774-014-0596-4. View

12.

. Osteoporosis prevention, diagnosis, and therapy. JAMA. 2001; 285(6):785-95. DOI: 10.1001/jama.285.6.785. View

13.

Weibel E, KISTLER G, Scherle W . Practical stereological methods for morphometric cytology. J Cell Biol. 1966; 30(1):23-38. PMC: 2106982. DOI: 10.1083/jcb.30.1.23. View

14.

Ardawi M, Badawoud M, Hassan S, Rouzi A, Ardawi J, ALNosani N . Lycopene treatment against loss of bone mass, microarchitecture and strength in relation to regulatory mechanisms in a postmenopausal osteoporosis model. Bone. 2015; 83:127-140. DOI: 10.1016/j.bone.2015.10.017. View

15.

Costa-Rodrigues J, Fernandes M, Pinho O, Monteiro P . Modulation of human osteoclastogenesis and osteoblastogenesis by lycopene. J Nutr Biochem. 2018; 57:26-34. DOI: 10.1016/j.jnutbio.2018.03.004. View

16.

Mackinnon E, Rao A, Josse R, Rao L . Supplementation with the antioxidant lycopene significantly decreases oxidative stress parameters and the bone resorption marker N-telopeptide of type I collagen in postmenopausal women. Osteoporos Int. 2010; 22(4):1091-101. DOI: 10.1007/s00198-010-1308-0. View

17.

Kalu D . The ovariectomized rat model of postmenopausal bone loss. Bone Miner. 1991; 15(3):175-91. DOI: 10.1016/0169-6009(91)90124-i. View

18.

Rufato F, Sousa L, Scalize P, Gimenes R, Hallak Regalo I, Rosa A . Texturized P(VDF-TrFE)/BT membrane enhances bone neoformation in calvaria defects regardless of the association with photobiomodulation therapy in ovariectomized rats. Clin Oral Investig. 2021; 26(1):1053-1065. DOI: 10.1007/s00784-021-04089-y. View

19.

Sharma D, Ciani C, Marin P, Levy J, Doty S, Fritton S . Alterations in the osteocyte lacunar-canalicular microenvironment due to estrogen deficiency. Bone. 2012; 51(3):488-97. PMC: 3412941. DOI: 10.1016/j.bone.2012.05.014. View

20.

Li X, Xue W, Cao Y, Long Y, Xie M . Effect of lycopene on titanium implant osseointegration in ovariectomized rats. J Orthop Surg Res. 2018; 13(1):237. PMC: 6142359. DOI: 10.1186/s13018-018-0944-5. View