A Dual-function SNF2 Protein Drives Chromatid Resolution and Nascent Transcripts Removal in Mitosis

Overview

Journal EMBO Rep

Specialty Molecular Biology

Date 2023 Jul 18

PMID 37462213

Authors

Catarina Carmo

Joao Coelho

Rui D Silva

Alexandra Tavares

Ana Boavida

Paola Gaetani

Leonardo G Guilgur

Rui Goncalo Martinho

Raquel A Oliveira

Affiliations

Soon will be listed here.

Abstract

Mitotic chromatin is largely assumed incompatible with transcription due to changes in the transcription machinery and chromosome architecture. However, the mechanisms of mitotic transcriptional inactivation and their interplay with chromosome assembly remain largely unknown. By monitoring ongoing transcription in Drosophila early embryos, we reveal that eviction of nascent mRNAs from mitotic chromatin occurs after substantial chromosome compaction and is not promoted by condensin I. Instead, we show that the timely removal of transcripts from mitotic chromatin is driven by the SNF2 helicase-like protein Lodestar (Lds), identified here as a modulator of sister chromatid cohesion defects. In addition to the eviction of nascent transcripts, we uncover that Lds cooperates with Topoisomerase 2 to ensure efficient sister chromatid resolution and mitotic fidelity. We conclude that the removal of nascent transcripts upon mitotic entry is not a passive consequence of cell cycle progression and/or chromosome compaction but occurs via dedicated mechanisms with functional parallelisms to sister chromatid resolution.

Citing Articles

A dual-function SNF2 protein drives chromatid resolution and nascent transcripts removal in mitosis.

Carmo C, Coelho J, Silva R, Tavares A, Boavida A, Gaetani P EMBO Rep. 2023; 24(9):e56463.

PMID: 37462213 PMC: 10481674. DOI: 10.15252/embr.202256463.

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