Structural Characterization and Antioxidant Activity of Pectic Polysaccharides from L

Overview

Journal Front Nutr

Specialty Nutritional Sciences

Date 2023 Jul 17

PMID 37457979

Authors

Su Yan

Xianbin Liu

Yuwen Wang

Xiaomin Yang

Lu Bai

Lin Sun

Yifa Zhou

Sisi Cui

Affiliations

Soon will be listed here.

Abstract

Background: Pectins are a class of acidic polysaccharides with complex structures. Different pectin molecules are composed of different domains, which have an important impact on their biological activity.

Objective: This study aimed to determine the structural features and the antioxidant activities of the pectic polysaccharides isolated from L.

Methods: The polysaccharide was isolated from L by water extraction and fractionated by ion exchange chromatography and gel permeation chromatography. The structure features of the pectic polysaccharides were determined by Fourier transforminfrared spectroscopy (FT-IR) and Nuclear magnetic resonance (NMR). The antioxidant activities was evaluated by the DPPH, OH and ABTS radical scavenging ability.

Results: WVPP-A2b and WVPP-A3b, with molecular weights of 48.7 × 10 and 77.6 × 10 kDa, respectively, contained homogalacturonan (HG), rhamnogalacturonan I (RG-I), and rhamnogalacturonan II (RG-II) domains with a mass ratio of 2.08:2.64:1.00 and 3.87:4.65:1:00, respectively. The RG-I domain contained an arabinogalactan II backbone and arabinans consisting of t-Ara, (1→5)-α-Ara, and (1→3,5)-α-Ara. WVPP-A3b also contained short chains consisting of the [t-Ara-(1→5)-α-Ara-(1→] structural unit. WVPP-A3b showed stronger ability to scavenge DPPH, hydroxyl, and ABTS radicals, which was potentially associated with its high content of galacturonic acid and presence of the HG domain.

Conclusion: The results provide information for enhancing knowledge of the structureactivity relationship of pectic polysaccharides from and their potential application in the healthcare food field.

Citing Articles

Family Malvaceae: a potential source of secondary metabolites with chemopreventive and anticancer activities supported with pharmacokinetic and pharmacodynamic profiles.

Sameh S, Elissawy A, Al-Sayed E, M Labib R, Chang H, Yu S Front Pharmacol. 2024; 15:1465055.

PMID: 39478959 PMC: 11521888. DOI: 10.3389/fphar.2024.1465055.

References

Li N, Liu X, He X, Wang S, Cao S, Xia Z . Structure and anticoagulant property of a sulfated polysaccharide isolated from the green seaweed Monostroma angicava. Carbohydr Polym. 2017; 159:195-206. DOI: 10.1016/j.carbpol.2016.12.013. View

Linhart Y . INTRA-POPULATION DIFFERENTIATION IN ANNUAL PLANTS I. VERONICA PEREGRINA L. RAISED UNDER NON-COMPETITIVE CONDITIONS. Evolution. 2017; 28(2):232-243. DOI: 10.1111/j.1558-5646.1974.tb00742.x. View

Chen X, Qi Y, Zhu C, Wang Q . Effect of ultrasound on the properties and antioxidant activity of hawthorn pectin. Int J Biol Macromol. 2019; 131:273-281. DOI: 10.1016/j.ijbiomac.2019.03.077. View

Ning X, Liu Y, Jia M, Wang Q, Sun Z, Ji L . Pectic polysaccharides from Radix Sophorae Tonkinensis exhibit significant antioxidant effects. Carbohydr Polym. 2021; 262:117925. DOI: 10.1016/j.carbpol.2021.117925. View

Halliwell B, Gutteridge J . Oxygen toxicity, oxygen radicals, transition metals and disease. Biochem J. 1984; 219(1):1-14. PMC: 1153442. DOI: 10.1042/bj2190001. View

Ojha A, Maiti D, Chandra K, Mondal S, Das Sadhan K Roy D, Ghosh K . Structural assignment of a heteropolysaccharide isolated from the gum of Cochlospermum religiosum (Katira gum). Carbohydr Res. 2008; 343(7):1222-31. DOI: 10.1016/j.carres.2008.03.010. View

Li Y, Lin D, Jiao B, Xu C, Qin J, Ye G . Purification, antioxidant and hepatoprotective activities of polysaccharide from Cissus pteroclada Hayata. Int J Biol Macromol. 2015; 77:307-13. DOI: 10.1016/j.ijbiomac.2015.03.039. View

Naran R, Chen G, Carpita N . Novel rhamnogalacturonan I and arabinoxylan polysaccharides of flax seed mucilage. Plant Physiol. 2008; 148(1):132-41. PMC: 2528086. DOI: 10.1104/pp.108.123513. View

Wikiera A, Koziol A, Mika M, Stodolak B . Structure and bioactivity of apple pectin isolated with arabinanase and mannanase. Food Chem. 2022; 388:133020. DOI: 10.1016/j.foodchem.2022.133020. View

10.

Yang B, Prasad K, Jiang Y . Structure identification of a polysaccharide purified from litchi (Litchi chinensis Sonn.) pulp. Carbohydr Polym. 2015; 137:570-575. DOI: 10.1016/j.carbpol.2015.10.088. View

11.

He T, Huang Y, Huang Y, Wang X, Hu J, Sheng J . Structural elucidation and antioxidant activity of an arabinogalactan from the leaves of Moringa oleifera. Int J Biol Macromol. 2018; 112:126-133. DOI: 10.1016/j.ijbiomac.2018.01.110. View

12.

Guo R, Tian S, Li X, Wu X, Liu X, Li D . Pectic polysaccharides from purple passion fruit peel: A comprehensive study in macromolecular and conformational characterizations. Carbohydr Polym. 2019; 229:115406. DOI: 10.1016/j.carbpol.2019.115406. View

13.

Zhu Z, Song X, Jiang Y, Yao J, Jiang Y, Li Z . Chemical structure and antioxidant activity of a neutral polysaccharide from Asteris Radix et Rhizoma. Carbohydr Polym. 2022; 286:119309. DOI: 10.1016/j.carbpol.2022.119309. View

14.

BLUMENKRANTZ N, ASBOE-HANSEN G . New method for quantitative determination of uronic acids. Anal Biochem. 1973; 54(2):484-9. DOI: 10.1016/0003-2697(73)90377-1. View

15.

Kwak J, Kim H, Lee K, Kang S, Zee O . Antioxidative iridoid glycosides and phenolic compounds from Veronica peregrina. Arch Pharm Res. 2009; 32(2):207-13. DOI: 10.1007/s12272-009-1137-x. View

16.

Qu Y, Yan J, Zhang X, Song C, Zhang M, Mayo K . Structure and antioxidant activity of six mushroom-derived heterogalactans. Int J Biol Macromol. 2022; 209(Pt A):1439-1449. DOI: 10.1016/j.ijbiomac.2022.04.135. View

17.

Yang X, Wang R, Zhang S, Zhu W, Tang J, Liu J . Polysaccharides from Panax japonicus C.A. Meyer and their antioxidant activities. Carbohydr Polym. 2013; 101:386-91. DOI: 10.1016/j.carbpol.2013.09.038. View

18.

Liu Y, Zhang B, Ibrahim S, Gao S, Yang H, Huang W . Purification, characterization and antioxidant activity of polysaccharides from Flammulina velutipes residue. Carbohydr Polym. 2016; 145:71-7. DOI: 10.1016/j.carbpol.2016.03.020. View

19.

Wang J, Hu S, Nie S, Yu Q, Xie M . Reviews on Mechanisms of In Vitro Antioxidant Activity of Polysaccharides. Oxid Med Cell Longev. 2015; 2016:5692852. PMC: 4670676. DOI: 10.1155/2016/5692852. View