Molecular Characterization and Marker Development of the Gene from for Improving Wheat Quality

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2023 Jul 14

PMID 37446250

Authors

Yi Dai

Jinfeng Li

Juntao Shi

Yujiao Gao

Haigang Ma

Yonggang Wang

Hongxiang Ma

Affiliations

Soon will be listed here.

Abstract

The quality of wheat primarily depends on its storage protein quality, especially in regards to gluten content and high-molecular-weight glutenin subunits (HMW-GS). The number of HMW-GS alleles is limited in bread wheat ( L.), whereas it is abundant in wheat relatives. Therefore, HMW-GS alleles from wheat relatives could provide a potential for improving quality in wheat breeding. (EE) is one of the relatives of wheat. The E genome is closely related to the ABD genome in wheat; therefore, is often used as an excellent exogenous gene donor for wheat genetic improvement. In this study, the high-molecular glutenin subunit gene was cloned and sequenced from . A specific molecular marker for identifying the subunit gene was developed and applied to detected wheat- alien introgression lines. Quality analysis indicated that the substitution and addition lines containing alleles significantly ( < 0.05) increased grain protein content by 3.76% to 5.11%, wet-gluten content by 6.55% to 8.73%, flour 8-MW by 0.25% to 6.35%, and bread volume value by 33.77 mL to 246.50 mL, in comparing it with Chinese Spring. The GMP content and lactic acid SRC showed significant positive correlations with flour processing quality and might be used as indicators for wheat quality. The results were expected to provide a novel route for improving processing quality in wheat quality breeding.

References

Zhou J, Yao C, Yang E, Yin M, Liu C, Ren Z . Characterization of a new wheat-Aegilops biuncialis addition line conferring quality-associated HMW glutenin subunits. Genet Mol Res. 2014; 13(1):660-9. DOI: 10.4238/2014.January.28.11. View

Du X, Jia Z, Yu Y, Wang S, Che B, Ni F . A wheat- addition line improves wheat agronomic traits and processing quality. Breed Sci. 2019; 69(3):503-507. PMC: 6776148. DOI: 10.1270/jsbbs.18200. View

Wei J, Geng H, Zhang Y, Liu J, Wen W, Zhang Y . Mapping quantitative trait loci for peroxidase activity and developing gene-specific markers for TaPod-A1 on wheat chromosome 3AL. Theor Appl Genet. 2015; 128(10):2067-76. DOI: 10.1007/s00122-015-2567-0. View

Singh J, Sheikh I, Sharma P, Kumar S, Verma S, Kumar R . Transfer of HMW glutenin subunits from to wheat through radiation hybridization. J Food Sci Technol. 2016; 53(9):3543-3549. PMC: 5069258. DOI: 10.1007/s13197-016-2333-6. View

Kumar A, Kapoor P, Chunduri V, Sharma S, Garg M . Potential of sp. for Improvement of Grain Processing and Nutritional Quality in Wheat (). Front Plant Sci. 2019; 10:308. PMC: 6431632. DOI: 10.3389/fpls.2019.00308. View

Borrajo C, Sanchez-Moreiras A, Reigosa M . Ecophysiological Responses of Tall Wheatgrass Germplasm to Drought and Salinity. Plants (Basel). 2022; 11(12). PMC: 9227858. DOI: 10.3390/plants11121548. View

Wang H, Sun S, Ge W, Zhao L, Hou B, Wang K . Horizontal gene transfer of from fungus underlies head blight resistance in wheat. Science. 2020; 368(6493). DOI: 10.1126/science.aba5435. View

Payne P, Holt L, Law C . Structural and genetical studies on the high-molecular-weight subunits of wheat glutenin : Part 1: Allelic variation in subunits amongst varieties of wheat (Triticum aestivum). Theor Appl Genet. 2013; 60(4):229-36. DOI: 10.1007/BF02342544. View

Li W, Wan Y, Liu Z, Liu K, Liu X, Li B . Molecular characterization of HMW glutenin subunit allele 1Bx14: further insights in to the evolution of Glu-B1-1 alleles in wheat and related species. Theor Appl Genet. 2004; 109(5):1093-104. DOI: 10.1007/s00122-004-1726-5. View

10.

Chang J, Zhang J, Mao X, Li A, Jia J, Jing R . Polymorphism of TaSAP1-A1 and its association with agronomic traits in wheat. Planta. 2013; 237(6):1495-508. DOI: 10.1007/s00425-013-1860-x. View

11.

Bagge M, Xia X, Lubberstedt T . Functional markers in wheat. Curr Opin Plant Biol. 2007; 10(2):211-6. DOI: 10.1016/j.pbi.2007.01.009. View

12.

Delorean E, Gao L, Cervantes Lopez J, Wulff B, Ibba M, Poland J . High molecular weight glutenin gene diversity in Aegilops tauschii demonstrates unique origin of superior wheat quality. Commun Biol. 2021; 4(1):1242. PMC: 8560932. DOI: 10.1038/s42003-021-02563-7. View

13.

Thompson J, Gibson T, Plewniak F, Jeanmougin F, Higgins D . The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 1998; 25(24):4876-82. PMC: 147148. DOI: 10.1093/nar/25.24.4876. View

14.

Halford N, Field J, Blair H, Urwin P, Moore K, Robert L . Analysis of HMW glutenin subunits encoded by chromosome 1A of bread wheat (Triticum aestivum L.) indicates quantitative effects on grain quality. Theor Appl Genet. 2013; 83(3):373-8. DOI: 10.1007/BF00224285. View

15.

Liu Z, Yan Z, Wan Y, Liu K, Zheng Y, Wang D . Analysis of HMW glutenin subunits and their coding sequences in two diploid Aegilops species. Theor Appl Genet. 2003; 106(8):1368-78. DOI: 10.1007/s00122-002-1175-y. View

16.

Roy N, Islam S, Al-Habbar Z, Yu Z, Liu H, Lafiandra D . Contribution to Breadmaking Performance of Two Different HMW Glutenin Alleles Expressed in Hexaploid Wheat. J Agric Food Chem. 2020; 69(1):36-44. DOI: 10.1021/acs.jafc.0c03880. View

17.

Li Y, Fu J, Shen Q, Yang D . High-Molecular-Weight Glutenin Subunits: Genetics, Structures, and Relation to End Use Qualities. Int J Mol Sci. 2020; 22(1). PMC: 7795185. DOI: 10.3390/ijms22010184. View

18.

Lawrence G, Shepherd K . Chromosomal location of genes controlling seed proteins in species related to wheat. Theor Appl Genet. 2013; 59(1):25-31. DOI: 10.1007/BF00275771. View

19.

Song F, Ni J, Qian Y, Li L, Ni D, Yang J . Development of SNP-based dCAPS markers for identifying male sterile gene tms5 in two-line hybrid rice. Genet Mol Res. 2016; 15(3). DOI: 10.4238/gmr.15038512. View

20.

Zeng J, Zhou C, He Z, Wang Y, Xu L, Chen G . Disomic Substitution of 3D Chromosome with Its Homoeologue 3E in Tetraploid Enhances Wheat Seedlings Tolerance to Salt Stress. Int J Mol Sci. 2023; 24(2). PMC: 9863149. DOI: 10.3390/ijms24021609. View