Analysis of HMW Glutenin Subunits Encoded by Chromosome 1A of Bread Wheat (Triticum Aestivum L.) Indicates Quantitative Effects on Grain Quality

Overview

Journal Theor Appl Genet

Publisher Springer

Specialty Genetics

Date 2013 Nov 9

PMID 24202521

Citations 42

Authors

N G Halford

J M Field

H Blair

P Urwin

K Moore

L Robert

R Thompson

R B Flavell

A S Tatham

P R Shewry

Affiliations

Soon will be listed here.

Abstract

A gene encoding the high-molecular-weight (HMW) subunit of glutenin 1Ax1 was isolated from bread wheat cv Hope. Comparison of the deduced amino acid sequence with that previously reported for an allelic subunit, 1Ax2(*), showed only minor differences, which were consistent with both subunits being associated with good bread-making quality. Quantitative analyses of total protein extracts from 22 cultivars of bread wheat showed that the presence of either subunit 1Ax1 or 1Ax2(*), when compared with a null allele, resulted in an increase in the proportion of HMW subunit protein from ca. 8 to 10% of the total. It is suggested that this quantitative increase in HMW subunit protein may account for the association of 1Ax subunits with good quality.

Citing Articles

Molecular Characterization and Marker Development of the Gene from for Improving Wheat Quality.

Dai Y, Li J, Shi J, Gao Y, Ma H, Wang Y Int J Mol Sci. 2023; 24(13).

PMID: 37446250 PMC: 10341674. DOI: 10.3390/ijms241311072.

Post-translational cleavage of HMW-GS allele improves the cookie-making quality in common wheat ().

Wang Y, Chen Q, Li Y, Guo Z, Liu C, Wan Y Mol Breed. 2023; 41(8):49.

PMID: 37309542 PMC: 10236088. DOI: 10.1007/s11032-021-01238-9.

Molecular Characterization and SNP-Based Molecular Marker Development of Two Novel High Molecular Weight Glutenin Genes from L.

Cao Y, Zhang J, Wang R, Sun H, Yan Y Int J Mol Sci. 2022; 23(19).

PMID: 36232404 PMC: 9570065. DOI: 10.3390/ijms231911104.

High-Molecular-Weight Glutenin Subunits: Genetics, Structures, and Relation to End Use Qualities.

Li Y, Fu J, Shen Q, Yang D Int J Mol Sci. 2020; 22(1).

PMID: 33375389 PMC: 7795185. DOI: 10.3390/ijms22010184.

A Cross between Bread Wheat and a 2D(2R) Disomic Substitution Triticale Line Leads to the Formation of a Novel Disomic Addition Line and Provides Information of the Role of Rye Secalins on Breadmaking Characteristics.

Sestili F, Margiotta B, Vaccino P, Moscaritolo S, Giorgi D, Lucretti S Int J Mol Sci. 2020; 21(22).

PMID: 33182791 PMC: 7696169. DOI: 10.3390/ijms21228450.

References

Sugiyama T, Rafalski A, Peterson D, Soll D . A wheat HMW glutenin subunit gene reveals a highly repeated structure. Nucleic Acids Res. 1985; 13(24):8729-37. PMC: 318947. DOI: 10.1093/nar/13.24.8729. View

Chou P, Fasman G . Empirical predictions of protein conformation. Annu Rev Biochem. 1978; 47:251-76. DOI: 10.1146/annurev.bi.47.070178.001343. View

Lawrence G, Shepherd K . Inheritance of glutenin protein subunits of wheat. Theor Appl Genet. 2013; 60(6):333-7. DOI: 10.1007/BF00264323. View

Payne P, Law C, Mudd E . Control by homoeologous group 1 chromosomes of the high-molecular-weight subunits of glutenin, a major protein of wheat endosperm. Theor Appl Genet. 2013; 58(3-4):113-20. DOI: 10.1007/BF00263101. View

Payne P, Corfield K, Blackman J . Identification of a high-molecular-weight subunit of glutenin whose presence correlates with bread-making quality in wheats of related pedigree. Theor Appl Genet. 2013; 55(3-4):153-9. DOI: 10.1007/BF00295442. View

Payne P, Holt L, Law C . Structural and genetical studies on the high-molecular-weight subunits of wheat glutenin : Part 1: Allelic variation in subunits amongst varieties of wheat (Triticum aestivum). Theor Appl Genet. 2013; 60(4):229-36. DOI: 10.1007/BF02342544. View

Thompson R, Bartels D, Harberd N, Flavell R . Characterization of the multigene family coding for HMW glutenin subunits in wheat using cDNA clones. Theor Appl Genet. 2013; 67(1):87-96. DOI: 10.1007/BF00303930. View

Harberd N, Bartels D, Thompson R . DNA restriction-fragment variation in the gene family encoding high molecular weight (HMW) glutenin subunits of wheat. Biochem Genet. 1986; 24(7-8):579-96. DOI: 10.1007/BF00504336. View

Devereux J, Haeberli P, SMITHIES O . A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984; 12(1 Pt 1):387-95. PMC: 321012. DOI: 10.1093/nar/12.1part1.387. View

10.

Rhodes C, Pierce D, Mettler I, Mascarenhas D, Detmer J . Genetically transformed maize plants from protoplasts. Science. 1988; 240(4849):204-7. DOI: 10.1126/science.2832947. View

11.

Anderson O, Greene F . The characterization and comparative analysis of high-molecular-weight glutenin genes from genomes A and B of a hexaploid bread wheat. Theor Appl Genet. 2013; 77(5):689-700. DOI: 10.1007/BF00261246. View

12.

Field J, Shewry P, Miflin B . Solubilisation and characterisation of wheat gluten proteins: correlations between the amount of aggregated proteins and baking quality. J Sci Food Agric. 1983; 34(4):370-7. DOI: 10.1002/jsfa.2740340409. View

13.

Gordon-Kamm W, Spencer T, Mangano M, Adams T, Daines R, Start W . Transformation of Maize Cells and Regeneration of Fertile Transgenic Plants. Plant Cell. 1990; 2(7):603-618. PMC: 159915. DOI: 10.1105/tpc.2.7.603. View

14.

Fromm M, Morrish F, Armstrong C, Williams R, Thomas J, Klein T . Inheritance and expression of chimeric genes in the progeny of transgenic maize plants. Biotechnology (N Y). 1990; 8(9):833-9. DOI: 10.1038/nbt0990-833. View