KCNE1 Does Not Shift TMEM16A from a Ca Dependent to a Voltage Dependent Cl Channel and is Not Expressed in Renal Proximal Tubule

Overview

Journal Pflugers Arch

Specialty Physiology

Date 2023 Jul 13

PMID 37442855

Authors

Khaoula Talbi

Jiraporn Ousingsawat

Raquel Centeio

Rainer Schreiber

Karl Kunzelmann

Affiliations

Soon will be listed here.

Abstract

The TMEM16A (ANO1) Cl channel is activated by Ca in a voltage-dependent manner. It is broadly expressed and was shown to be also present in renal proximal tubule (RPT). KCNQ1 is an entirely different K selective channel that forms the cardiac I potassium channel together with its ß-subunit KCNE1. Surprisingly, KCNE1 has been claimed to interact with TMEM16A, and to be required for activation of TMEM16A in mouse RPT. Interaction with KCNE1 was reported to switch TMEM16A from a Ca2-dependent to a voltage-dependent ion channel. Here we demonstrate that KCNE1 is not expressed in mouse RPT. TMEM16A expressed in RPT is activated by angiotensin II and ATP in a KCNE1-independent manner. Coexpression of KCNE1 does not change TMEM16A to a voltage gated Cl channel and Ca-dependent regulation of TMEM16A is fully maintained in the presence of KCNE1. While overexpressed KCNE1 slightly affects Ca-dependent regulation of TMEM16A, the data provide no evidence for KCNE1 being an auxiliary functional subunit for TMEM16A.

Citing Articles

Identification of KCNE6, a new member of the KCNE family of potassium channel auxiliary subunits.

Kasuya G, Zempo B, Yamamoto Y, Ryu K, Ono F, Nakajo K Commun Biol. 2024; 7(1):1662.

PMID: 39702752 PMC: 11659587. DOI: 10.1038/s42003-024-07352-6.

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