LncRNA SNHG20 Silencing Inhibits Hepatocellular Carcinoma Progression by Sponging MiR-5095 from MBD1

Overview

Journal Am J Transl Res

Specialty General Medicine

Date 2023 Jul 12

PMID 37434830

Authors

Bin Xu

Chao Li

Bin Yang

Fan Zhou

Kezhong Tang

Lantian Wang

Wenjie Lu

Affiliations

Soon will be listed here.

Abstract

Objective: Long non-coding RNAs (lncRNAs) may have a significant regulatory effect on the progression of hepatocellular carcinoma (HCC), according to recent data. This study aims to investigate how SNHG20, a small nucleolar RNA host gene, contributes to the development of HCC.

Methods: LncRNA SNHG20, miR-5095, and MBD1 gene levels were determined using reverse transcription qPCR (RT-qPCR). Huh-7 and HepG2 cell bioactivities were evaluated using the CCK-8 kit, EdU, flow cytometry, and wound-healing migration tests. To assess the metastasis of Huh-7 and HepG2 cells, a transwell assay was used. The amounts of invasion- and proliferation-associated proteins were determined using western blot. Using the miRDB (www.mirdb.org) software, the possible target genes of lncRNA and miRNA were predicted, and this prediction was then verified by a twofold luciferase reporter test. To determine the pathologic alteration and Ki67 level in tumor tissues, H&E staining and IHC were employed. TUNEL was conducted to assess the presence of apoptotic bodies in the tumor tissues.

Results: lncRNA SNHG20 exhibited a high expression in HCC cells (P<0.01). LncRNA SNHG20 knockdown inhibited HCC cell metastasis (P<0.01) and accelerated apoptosis (P<0.01). LncRNA SNHG20 acted as a sponge of miR-5095 in HCC. In addition, miR-5095 overexpression inhibited HCC cell metastasis (P<0.01) and accelerated apoptosis (P<0.01); and miR-5095 negatively targeted MBD1. Furthermore, LncRNA SNHG20 regulated HCC progression through the miR-5095/MBD1 axis, and LncRNA SNHG20 knockdown inhibited HCC growth.

Conclusion: LncRNA SNHG20 accelerates HCC progression by the miR-5095/MBD1 axis, indicating lncRNA SNHG20 can be used as a biomarker for patients with HCC.

Citing Articles

Exploring non-coding RNA mechanisms in hepatocellular carcinoma: implications for therapy and prognosis.

Tian Y, Zhang M, Liu L, Wang Z, Liu B, Huang Y Front Immunol. 2024; 15:1400744.

PMID: 38799446 PMC: 11116607. DOI: 10.3389/fimmu.2024.1400744.

References

Zhao H, Zheng G, Li G, Xin L, Wang Y, Chen Y . Long noncoding RNA LINC00958 regulates cell sensitivity to radiotherapy through RRM2 by binding to microRNA-5095 in cervical cancer. J Cell Physiol. 2019; 234(12):23349-23359. DOI: 10.1002/jcp.28902. View

Yang L, Peng X, Li Y, Zhang X, Ma Y, Wu C . Long non-coding RNA HOTAIR promotes exosome secretion by regulating RAB35 and SNAP23 in hepatocellular carcinoma. Mol Cancer. 2019; 18(1):78. PMC: 6446409. DOI: 10.1186/s12943-019-0990-6. View

Ding Y, Wang H, Shen H, Li Z, Geng J, Han H . The clinical pathology of severe acute respiratory syndrome (SARS): a report from China. J Pathol. 2003; 200(3):282-9. PMC: 7168017. DOI: 10.1002/path.1440. View

Kondo Y, Shinjo K, Katsushima K . Long non-coding RNAs as an epigenetic regulator in human cancers. Cancer Sci. 2017; 108(10):1927-1933. PMC: 5623749. DOI: 10.1111/cas.13342. View

Elton T, Selemon H, Elton S, Parinandi N . Regulation of the MIR155 host gene in physiological and pathological processes. Gene. 2012; 532(1):1-12. DOI: 10.1016/j.gene.2012.12.009. View

Ren Y, Wang Y, Hao S, Yang Y, Xiong W, Qiu L . NFE2L3 promotes malignant behavior and EMT of human hepatocellular carcinoma (HepG2) cells via Wnt/β‑catenin pathway. J Cancer. 2020; 11(23):6939-6949. PMC: 7591994. DOI: 10.7150/jca.48100. View

Li Y, Tian Y, Zhong W, Wang N, Wang Y, Zhang Y . Essential Oil Inhibits Hepatocellular Carcinoma Metastasis via Suppression of DEPDC1 Dependent Wnt/β-Catenin Signaling Pathway. Front Cell Dev Biol. 2021; 9:664791. PMC: 8276134. DOI: 10.3389/fcell.2021.664791. View

Dong S, Cui J, Zhang W, Zhang X, Kou T, Cai Q . Inhibition of translation initiation factor eIF4A is required for apoptosis mediated by Microplitis bicoloratus bracovirus. Arch Insect Biochem Physiol. 2017; 96(3). DOI: 10.1002/arch.21423. View

Fang Y, Fullwood M . Roles, Functions, and Mechanisms of Long Non-coding RNAs in Cancer. Genomics Proteomics Bioinformatics. 2016; 14(1):42-54. PMC: 4792843. DOI: 10.1016/j.gpb.2015.09.006. View

10.

Huang M, Chen W, Qi F, Sun M, Xu T, Ma P . Long non-coding RNA TUG1 is up-regulated in hepatocellular carcinoma and promotes cell growth and apoptosis by epigenetically silencing of KLF2. Mol Cancer. 2015; 14:165. PMC: 4558931. DOI: 10.1186/s12943-015-0431-0. View

11.

Li L, Chen B, Chan W . An epigenetic regulator: methyl-CpG-binding domain protein 1 (MBD1). Int J Mol Sci. 2015; 16(3):5125-40. PMC: 4394467. DOI: 10.3390/ijms16035125. View

12.

Lan X, Liu X, Sun J, Yuan Q, Li J . CircRAD23B facilitates proliferation and invasion of esophageal cancer cells by sponging miR-5095. Biochem Biophys Res Commun. 2019; 516(2):357-364. DOI: 10.1016/j.bbrc.2019.06.044. View

13.

Wei J, Huang K, Yang C, Kang C . Non-coding RNAs as regulators in epigenetics (Review). Oncol Rep. 2016; 37(1):3-9. DOI: 10.3892/or.2016.5236. View

14.

Lai X, Li J, Tang L, Chen W, Zhang L, Xiong L . MiRNAs and LncRNAs: Dual Roles in TGF-β Signaling-Regulated Metastasis in Lung Cancer. Int J Mol Sci. 2020; 21(4). PMC: 7072809. DOI: 10.3390/ijms21041193. View

15.

Shi Y, Fang N, Li Y, Guo Z, Jiang W, He Y . Circular RNA LPAR3 sponges microRNA-198 to facilitate esophageal cancer migration, invasion, and metastasis. Cancer Sci. 2020; 111(8):2824-2836. PMC: 7419039. DOI: 10.1111/cas.14511. View

16.

Zhu Y, Shi C, Zeng L, Liu G, Jiang W, Zhang X . High COX-2 expression in cancer-associated fibiroblasts contributes to poor survival and promotes migration and invasiveness in nasopharyngeal carcinoma. Mol Carcinog. 2019; 59(3):265-280. PMC: 7027878. DOI: 10.1002/mc.23150. View

17.

Chen X, Du J, Jiang R, Li L . MicroRNA-214 inhibits the proliferation and invasion of lung carcinoma cells by targeting JAK1. Am J Transl Res. 2018; 10(4):1164-1171. PMC: 5934575. View

18.

Hu X, Duan L, Liu H, Zhang L . Long noncoding RNA LINC01296 induces non-small cell lung cancer growth and progression through sponging miR-5095. Am J Transl Res. 2019; 11(2):895-903. PMC: 6413248. View

19.

Zhang Y, Yuan J, Guo M, Xiang R, Xie T, Zhuang X . Upregulation of long intergenic non-coding RNA LINC00326 inhibits non-small cell lung carcinoma progression by blocking Wnt/β-catenin pathway through modulating the miR-657/dickkopf WNT signaling pathway inhibitor 2 axis. Biol Direct. 2023; 18(1):3. PMC: 9901116. DOI: 10.1186/s13062-023-00359-9. View

20.

Li Y, Xu J, Guo Y, Yang B . LncRNA SNHG20 promotes the development of laryngeal squamous cell carcinoma by regulating miR-140. Eur Rev Med Pharmacol Sci. 2019; 23(8):3401-3409. DOI: 10.26355/eurrev_201904_17704. View