Synergistic Effect of Arginine and Against Potassium Dichromate Induced-acute Liver and Kidney Injury in Rats: Role of INOS and TLR4/NF-κB Signaling Pathways

Objectives: Our study was conducted to evaluate the synergistic effect of arginine (ARG) and against potassium dichromate (K2Cr2O7) induced-acute hepatic and kidney injury.

Materials And Methods: Fifty male Wistar rats were divided into five groups. The control group received distilled water. The potassium dichromate group (PDC) received a single dose of PDC (20 mg/kg; SC). The arginine group (ARG) and group received either daily doses of ARG (100 mg/kg, PO) or (10 CFU/ml, PO) for 14 days. The combination group (ARG+) received daily doses of ARG (100 mg/kg) with (10 CFU/ml), orally for 14 days, before induction of acute liver and kidney injury. Forty eight hours after the last dose of PDC, serum biochemical indices, oxidative stress biomarkers, pro-inflammatory cytokines, histopathological and immunohistochemical analysis were evaluated.

Results: Combining ARG with restored the levels of serum hepatic & kidney enzymes, hepatic & renal oxidative stress biomarkers, and TLR 4/ NF-κB signaling pathway. Furthermore, they succeeded in decreasing the expression of iNOS and ameliorate the hepatic and renal markers of apoptosis: Caspase-3, Bax, and Bcl2.

Conclusion: This study depicts that combining ARG with exerted a new bacteriotherapy against hepatic and renal injury caused by PDC.