Current State of Molecular and Metabolic Strategies for the Improvement of L-asparaginase Expression in Heterologous Systems

Overview

Journal Front Pharmacol

Date 2023 Jul 10

PMID 37426818

Authors

Nicolas Lefin

Javiera Miranda

Jorge F Beltran

Lisandra Herrera Belen

Brian Effer

Adalberto Pessoa Jr

Jorge G Farias

Mauricio Zamorano

Affiliations

Soon will be listed here.

Abstract

Heterologous expression of L-asparaginase (L-ASNase) has become an important area of research due to its clinical and food industry applications. This review provides a comprehensive overview of the molecular and metabolic strategies that can be used to optimize the expression of L-ASNase in heterologous systems. This article describes various approaches that have been employed to increase enzyme production, including the use of molecular tools, strain engineering, and optimization. The review article highlights the critical role that rational design plays in achieving successful heterologous expression and underscores the challenges of large-scale production of L-ASNase, such as inadequate protein folding and the metabolic burden on host cells. Improved gene expression is shown to be achievable through the optimization of codon usage, synthetic promoters, transcription and translation regulation, and host strain improvement, among others. Additionally, this review provides a deep understanding of the enzymatic properties of L-ASNase and how this knowledge has been employed to enhance its properties and production. Finally, future trends in L-ASNase production, including the integration of CRISPR and machine learning tools are discussed. This work serves as a valuable resource for researchers looking to design effective heterologous expression systems for L-ASNase production as well as for enzymes production in general.

Citing Articles

Engineering and Expression Strategies for Optimization of L-Asparaginase Development and Production.

Shishparenok A, Gladilina Y, Zhdanov D Int J Mol Sci. 2023; 24(20).

PMID: 37894901 PMC: 10607044. DOI: 10.3390/ijms242015220.

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