Potential Diagnostic Biomarkers: 6 Cuproptosis- and Ferroptosis-related Genes Linking Immune Infiltration in Acute Myocardial Infarction

Overview

Journal Genes Immun

Date 2023 Jul 8

PMID 37422588

Authors

Mengdan Miao

Shanhu Cao

Yifei Tian

Da Liu

Lixia Chen

Qiaoying Chai

Mei Wei

Shaoguang Sun

Le Wang

Shuanli Xin

Gang Liu

Mingqi Zheng

Affiliations

Soon will be listed here.

Abstract

The current diagnostic biomarkers of acute myocardial infarction (AMI), troponins, lack specificity and exist as false positives in other non-cardiac diseases. Previous studies revealed that cuproptosis, ferroptosis, and immune infiltration are all involved in the development of AMI. We hypothesize that combining the analysis of cuproptosis, ferroptosis, and immune infiltration in AMI will help identify more precise diagnostic biomarkers. The results showed that a total of 19 cuproptosis- and ferroptosis-related genes (CFRGs) were differentially expressed between the healthy and AMI groups. Functional enrichment analysis showed that the differential CFRGs were mostly enriched in biological processes related to oxidative stress and the inflammatory response. The immune infiltration status analyzed by ssGSEA found elevated levels of macrophages, neutrophils, and CCR in AMI. Then, we screened 6 immune-related CFRGs (CXCL2, DDIT3, DUSP1, CDKN1A, TLR4, STAT3) to construct a nomogram for predicting AMI and validated it in the GSE109048 dataset. Moreover, we also identified 5 pivotal miRNAs and 10 candidate drugs that target the 6 feature genes. Finally, RT-qPCR analysis verified that all 6 feature genes were upregulated in both animals and patients. In conclusion, our study reveals the significance of immune-related CFRGs in AMI and provides new insights for AMI diagnosis and treatment.

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