Identification and Genetic Characteristics of Tusavirus in Fecal Samples of Patients with Chronic Diseases in Guangzhou, China

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2023 Jul 3

PMID 37396384

Authors

Huan He

Yongzhi Li

Jiaqi Chen

Juxian Xian

Liting Zheng

Hengbiao Sun

Shunchang Fan

Jiaqi Fu

Qiushuang Li

Caiyun Chen

Minyi Liang

Minyi Zhang

Ruojun Wu

Gang Xiao

Qing Chen

Affiliations

Soon will be listed here.

Abstract

Purpose: The Tunisian stool-associated parvovirus [Tusavirus (TuV)] is a novel member of the genus which may be linked to diarrhea. Herein, we investigated the prevalence of TuV in different populations and analyzed its genetic and bioinformatic characteristics.

Methods: This study was conducted in a tertiary hospital in Guangzhou (China) from February 2018 to July 2022. Demographic and clinical information and stool samples were collected from individuals who visited the hospital. ProtScale, SwissModel, Datamonkey, and other tools were used to analyze and predict the physicochemical parameters, tertiary structure, selection pressure, and B-cell epitopes of capsid viral protein 2 of TuV (VP2-TuV).

Results: A total of 3,837 participants were enrolled, among which two stool samples from patients with chronic illnesses were tested positive for TuV DNA. However, no positive sample was detected among patients with diarrhea. Two near-complete genome sequences were amplified. The genetic analysis revealed the presence of diversity among TuVs isolated from distinct host species. Bioinformatics analysis revealed that VP2-TuV exhibited hydrophilic properties and lacked transmembrane domains and signal peptides. The secondary structure of VP2-TuV was composed mainly of random coils and β-strands. Selective-pressure analysis of the VP2 region suggested that TuV primarily underwent negative selection during evolution. Negatively selected codon sites coincided with residues comprising of B-cell epitopes, suggesting minimal changes in the immunogenicity of TuV over time.

Conclusion: TuV was detected in patients with chronic diseases but not in patients with diarrhea. The putative roles of TuV in the pathogenicity of human diseases and zoonotic viruses must be determined by additional studies.

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References

Fujishima Y, Nishiumi S, Masuda A, Inoue J, Nguyen N, Irino Y . Autophagy in the intestinal epithelium reduces endotoxin-induced inflammatory responses by inhibiting NF-κB activation. Arch Biochem Biophys. 2010; 506(2):223-35. DOI: 10.1016/j.abb.2010.12.009. View

Mietzsch M, McKenna R, Vaisanen E, Yu J, Ilyas M, Hull J . Structural Characterization of Cuta- and Tusavirus: Insight into Protoparvoviruses Capsid Morphology. Viruses. 2020; 12(6). PMC: 7354515. DOI: 10.3390/v12060653. View

Ghozlane A, Joseph A, Bornot A, de Brevern A . Analysis of protein chameleon sequence characteristics. Bioinformation. 2009; 3(9):367-9. PMC: 2732029. DOI: 10.6026/97320630003367. View

Mietzsch M, Penzes J, Agbandje-Mckenna M . . Viruses. 2019; 11(4). PMC: 6521121. DOI: 10.3390/v11040362. View

Jiang X, Huang P, Zhong W, Farkas T, Cubitt D, Matson D . Design and evaluation of a primer pair that detects both Norwalk- and Sapporo-like caliciviruses by RT-PCR. J Virol Methods. 1999; 83(1-2):145-54. DOI: 10.1016/s0166-0934(99)00114-7. View

Hafliger D, Gilgen M, Luthy J, Hubner P . Seminested RT-PCR systems for small round structured viruses and detection of enteric viruses in seafood. Int J Food Microbiol. 1997; 37(1):27-36. DOI: 10.1016/s0168-1605(97)00041-x. View

Blinkova O, Rosario K, Li L, Kapoor A, Slikas B, Bernardin F . Frequent detection of highly diverse variants of cardiovirus, cosavirus, bocavirus, and circovirus in sewage samples collected in the United States. J Clin Microbiol. 2009; 47(11):3507-13. PMC: 2772610. DOI: 10.1128/JCM.01062-09. View

Mohanraj U, Jokinen M, Rayamajhi Thapa R, Paloniemi M, Vesikari T, Lappalainen M . Human Protoparvovirus DNA and IgG in Children and Adults with and without Respiratory or Gastrointestinal Infections. Viruses. 2021; 13(3). PMC: 7999311. DOI: 10.3390/v13030483. View

Harvala H, Robertson I, McWilliam Leitch E, Benschop K, Wolthers K, Templeton K . Epidemiology and clinical associations of human parechovirus respiratory infections. J Clin Microbiol. 2008; 46(10):3446-53. PMC: 2566073. DOI: 10.1128/JCM.01207-08. View

10.

Noel J, Lee T, Kurtz J, Glass R, Monroe S . Typing of human astroviruses from clinical isolates by enzyme immunoassay and nucleotide sequencing. J Clin Microbiol. 1995; 33(4):797-801. PMC: 228043. DOI: 10.1128/jcm.33.4.797-801.1995. View

11.

Vennema H, de Bruin E, Koopmans M . Rational optimization of generic primers used for Norwalk-like virus detection by reverse transcriptase polymerase chain reaction. J Clin Virol. 2002; 25(2):233-5. DOI: 10.1016/s1386-6532(02)00126-9. View

12.

Miller P, Kim L, Ip H, Afonso C . Evolutionary dynamics of Newcastle disease virus. Virology. 2009; 391(1):64-72. DOI: 10.1016/j.virol.2009.05.033. View

13.

Kumthip K, Khamrin P, Ushijima H, Maneekarn N . Enteric and non-enteric adenoviruses associated with acute gastroenteritis in pediatric patients in Thailand, 2011 to 2017. PLoS One. 2019; 14(8):e0220263. PMC: 6675392. DOI: 10.1371/journal.pone.0220263. View

14.

Reuter G, Pankovics P, Laszlo Z, Gaspar G, Hui A, Delwart E . Human-stool-associated tusavirus (Parvoviridae) in domestic goats and sheep. Arch Virol. 2022; 167(5):1307-1310. PMC: 9038789. DOI: 10.1007/s00705-022-05424-8. View

15.

Lewis S, Heaton K . Stool form scale as a useful guide to intestinal transit time. Scand J Gastroenterol. 1997; 32(9):920-4. DOI: 10.3109/00365529709011203. View

16.

Hashemzadeh M, Mousavy S, Dorostkar R, Fotouhi F, Ebrahimi F . Designing Two Individual AcMNPV Polyhedrin-Plus Bac-to-Bac Expression System in order to Express GFP and CPV-VP2 in Insect Cells. Iran J Biotechnol. 2018; 15(3):172-178. PMC: 5811064. DOI: 10.15171/ijb.1558. View

17.

Kluge M, Campos F, Tavares M, de Amorim D, Valdez F, Giongo A . Metagenomic Survey of Viral Diversity Obtained from Feces of Subantarctic and South American Fur Seals. PLoS One. 2016; 11(3):e0151921. PMC: 4795697. DOI: 10.1371/journal.pone.0151921. View

18.

Huang Y, Bao Y, Guo S, Wang Y, Zhou C, Li Y . Pep-3D-Search: a method for B-cell epitope prediction based on mimotope analysis. BMC Bioinformatics. 2008; 9:538. PMC: 2639436. DOI: 10.1186/1471-2105-9-538. View

19.

Sivalingam G, Shepherd A . An analysis of B-cell epitope discontinuity. Mol Immunol. 2012; 51(3-4):304-9. PMC: 3657695. DOI: 10.1016/j.molimm.2012.03.030. View

20.

Phan T, Sdiri-Loulizi K, Aouni M, Ambert-Balay K, Pothier P, Deng X . New parvovirus in child with unexplained diarrhea, Tunisia. Emerg Infect Dis. 2014; 20(11):1911-3. PMC: 4214302. DOI: 10.3201/eid2011.140428. View