Changes in Glycosylated Proteins in Colostrum and Mature Milk and Their Implication

Overview

Journal Front Nutr

Specialty Nutritional Sciences

Date 2023 Jul 3

PMID 37396121

Authors

Jing Lu

Wenyuan Zhang

Changlu Ma

Xiaoyang Pang

Ying Dai

Tong Zhu

Jinqi Liu

Lina Xing

Shuwen Zhang

Jiaping Lv

Affiliations

Soon will be listed here.

Abstract

Introduction: Glycosylation is one of the essential post-translational modifications that influences the function of milk proteins.

Methods: In the present study, 998 proteins and 764 glycosylated sites from 402 glycoproteins were identified in human milk by TMT labeling proteomics. Compared to human milk proteins, the glycoproteins were mainly enriched in cell adhesion, proteolysis, and defense/immune process.

Results: The abundance of 353 glycosylated sites and their 179 parent proteins was quantified. After normalization to their parent protein's abundance, 78 glycosylated sites in 56 glycoproteins and 10 glycosylated sites in 10 glycoproteins were significantly higher in colostrum and mature milk, respectively. These changed glycoproteins were mainly related to host defense. Intriguingly, one glycosylated site (Asp144) in IgA and two glycosylated sites (Asp38 and Asp1079) in tenascin are significantly upregulated even though their protein abundance was downregulated during lactation.

Discussion: This study helps us figure out the critical glycosylated sites in proteins that might influence their biological function in an unbiased way.

Citing Articles

Bioactive Peptides in Greek Goat Colostrum: Relevance to Human Metabolism.

Petre M, Kontouli Pertesi A, Boulioglou O, Sarantidi E, Korovesi A, Kozei A Foods. 2024; 13(23).

PMID: 39683021 PMC: 11640771. DOI: 10.3390/foods13233949.

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