Progress and Promise of Alternative Animal and Non-animal Methods in Biomedical Research

Overview

Journal Arch Toxicol

Specialty Toxicology

Date 2023 Jul 2

PMID 37394624

Authors

Irlan Almeida Freires

David Fernando Colon Morelo

Lelio Fernando Ferreira Soares

Isabela Silva Costa

Leonardo Pereira de Araujo

Isadora Breseghello

Henrique Ballassini Abdalla

Josy Goldoni Lazarini

Pedro Luiz Rosalen

Suzane Cristina Pigossi

Marcelo Franchin

Affiliations

Soon will be listed here.

Abstract

Cell culture and invertebrate animal models reflect a significant evolution in scientific research by providing reliable evidence on the physiopathology of diseases, screening for new drugs, and toxicological tests while reducing the need for mammals. In this review, we discuss the progress and promise of alternative animal and non-animal methods in biomedical research, with a special focus on drug toxicity.

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References

Adamski Z, Bufo S, Chowanski S, Falabella P, Lubawy J, Marciniak P . Beetles as Model Organisms in Physiological, Biomedical and Environmental Studies - A Review. Front Physiol. 2019; 10:319. PMC: 6447812. DOI: 10.3389/fphys.2019.00319. View

Affleck J, Walker V . Drosophila as a Model for Developmental Toxicology: Using and Extending the Drosophotoxicology Model. Methods Mol Biol. 2019; 1965:139-153. DOI: 10.1007/978-1-4939-9182-2_10. View

Andersen M, Winter L . Animal models in biological and biomedical research - experimental and ethical concerns. An Acad Bras Cienc. 2017; 91(suppl 1):e20170238. DOI: 10.1590/0001-3765201720170238. View

Archer C, Sargeant R, Basak J, Pilling J, Barnes J, Pointon A . Characterization and Validation of a Human 3D Cardiac Microtissue for the Assessment of Changes in Cardiac Pathology. Sci Rep. 2018; 8(1):10160. PMC: 6033897. DOI: 10.1038/s41598-018-28393-y. View

Ates M, Demir V, Arslan Z, Daniels J, Farah I, Bogatu C . Evaluation of alpha and gamma aluminum oxide nanoparticle accumulation, toxicity, and depuration in Artemia salina larvae. Environ Toxicol. 2014; 30(1):109-18. PMC: 4006351. DOI: 10.1002/tox.21917. View

Augustyniak M, Gladysz M, Dziewiecka M . The Comet assay in insects--Status, prospects and benefits for science. Mutat Res Rev Mutat Res. 2016; 767:67-76. DOI: 10.1016/j.mrrev.2015.09.001. View

Banti C, Hadjikakou S . Evaluation of Toxicity with Brine Shrimp Assay. Bio Protoc. 2021; 11(2):e3895. PMC: 7952950. DOI: 10.21769/BioProtoc.3895. View

Bar-Ephraim Y, Kretzschmar K, Clevers H . Organoids in immunological research. Nat Rev Immunol. 2019; 20(5):279-293. DOI: 10.1038/s41577-019-0248-y. View

Belair D, Visconti R, Hong M, Marella M, Peters M, Scott C . Human ileal organoid model recapitulates clinical incidence of diarrhea associated with small molecule drugs. Toxicol In Vitro. 2020; 68:104928. DOI: 10.1016/j.tiv.2020.104928. View

10.

Belfiore L, Aghaei B, Law A, Dobrowolski J, Raftery L, Tjandra A . Generation and analysis of 3D cell culture models for drug discovery. Eur J Pharm Sci. 2021; 163:105876. DOI: 10.1016/j.ejps.2021.105876. View

11.

Bhise N, Ribas J, Manoharan V, Zhang Y, Polini A, Massa S . Organ-on-a-chip platforms for studying drug delivery systems. J Control Release. 2014; 190:82-93. PMC: 4142092. DOI: 10.1016/j.jconrel.2014.05.004. View

12.

Bielecka Z, Maliszewska-Olejniczak K, Safir I, Szczylik C, Czarnecka A . Three-dimensional cell culture model utilization in cancer stem cell research. Biol Rev Camb Philos Soc. 2016; 92(3):1505-1520. DOI: 10.1111/brv.12293. View

13.

Bircsak K, DeBiasio R, Miedel M, Alsebahi A, Reddinger R, Saleh A . A 3D microfluidic liver model for high throughput compound toxicity screening in the OrganoPlate®. Toxicology. 2020; 450:152667. DOI: 10.1016/j.tox.2020.152667. View

14.

Blaxter M . Nematodes: the worm and its relatives. PLoS Biol. 2011; 9(4):e1001050. PMC: 3079589. DOI: 10.1371/journal.pbio.1001050. View

15.

Boretto M, Maenhoudt N, Luo X, Hennes A, Boeckx B, Bui B . Patient-derived organoids from endometrial disease capture clinical heterogeneity and are amenable to drug screening. Nat Cell Biol. 2019; 21(8):1041-1051. DOI: 10.1038/s41556-019-0360-z. View

16.

Calap-Quintana P, Gonzalez-Fernandez J, Sebastia-Ortega N, Llorens J, Molto M . Drosophila melanogaster Models of Metal-Related Human Diseases and Metal Toxicity. Int J Mol Sci. 2017; 18(7). PMC: 5535947. DOI: 10.3390/ijms18071456. View

17.

Chan W, Shaughnessy A, van den Berg C, Garson M, Cheney K . The Validity of Brine Shrimp (Artemia Sp.) Toxicity Assays to Assess the Ecological Function of Marine Natural Products. J Chem Ecol. 2021; 47(10-11):834-846. DOI: 10.1007/s10886-021-01264-z. View

18.

Chifiriuc M, Ratiu A, Popa M, Ecovoiu A . Drosophotoxicology: An Emerging Research Area for Assessing Nanoparticles Interaction with Living Organisms. Int J Mol Sci. 2016; 17(2):36. PMC: 4783871. DOI: 10.3390/ijms17020036. View

19.

Coates C, Lim J, Harman K, Rowley A, Griffiths D, Emery H . The insect, Galleria mellonella, is a compatible model for evaluating the toxicology of okadaic acid. Cell Biol Toxicol. 2018; 35(3):219-232. PMC: 6556153. DOI: 10.1007/s10565-018-09448-2. View

20.

Cole R, Anderson G, Williams P . The nematode Caenorhabditis elegans as a model of organophosphate-induced mammalian neurotoxicity. Toxicol Appl Pharmacol. 2004; 194(3):248-56. DOI: 10.1016/j.taap.2003.09.013. View