Extraction, Rheological, and Physicochemical Properties of Water-Soluble Polysaccharides with Antioxidant Capacity from Pursh

Overview

Journal Foods

Specialty Biotechnology

Date 2023 Jun 28

PMID 37372546

Authors

Yi Chen

Li Song

Pei Chen

Huiping Liu

Xiaowei Zhang

Affiliations

Soon will be listed here.

Abstract

This study aimed to isolate polysaccharides from Pursh and evaluate their rheological characteristics, physicochemical properties, and antioxidant activity. The optimal conditions for the maximal extraction yield of Pursh polysaccharides (4.05 ± 0.12%) were determined by employing a single-factor test and response surface methodology which included an extraction time of 3 h, a liquid-solid ratio of 20 mL/g, and three separate extraction times. The rheological experiments showcased that the polysaccharides exhibited typical shear-thinning behavior, with their apparent viscosity being influenced by various parameters such as concentration, pH, temperature, salt content, and freeze-thaw. The purified polysaccharides (PCP-100), having an average molecular weight of 1.46 × 10 Da, mainly consisted of glucose (18.99%), arabinose (22.87%), galactose (26.72%), and galacturonic acid (21.89%). Furthermore, the PCP-100 exhibited high thermal stability and displayed an irregular sheet-like morphology. Its superior reducing power and free radical scavenging ability implied its significant antioxidant activity in vitro. Collectively, these findings provide important insights for the future application of polysaccharides in the food industry.

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