Mechanism of Inhibiting the Growth and Aflatoxin B Biosynthesis of by Phenyllactic Acid

Overview

Journal Toxins (Basel)

Publisher MDPI

Specialty Toxicology

Date 2023 Jun 27

PMID 37368671

Authors

Chi Zhao

Petri Penttinen

Lingzi Zhang

Ling Dong

Fengju Zhang

Zhihua Li

Xiaoping Zhang

Affiliations

Soon will be listed here.

Abstract

Phenyllactic acid (PLA), a promising food preservative, is safe and effective against a broad spectrum of food-borne pathogens. However, its mechanisms against toxigenic fungi are still poorly understood. In this study, we applied physicochemical, morphological, metabolomics, and transcriptomics analyses to investigate the activity and mechanism of PLA inhibition of a typical food-contaminating mold, . The results showed that PLA effectively inhibited the growth of spores and reduced aflatoxin B (AFB) production by downregulating key genes associated with AFB biosynthesis. Propidium iodide staining and transmission electron microscopy analysis demonstrated a dose-dependent disruption of the integrity and morphology of the spore cell membrane by PLA. Multi-omics analyses showed that subinhibitory concentrations of PLA induced significant changes in spores at the transcriptional and metabolic levels, as 980 genes and 30 metabolites were differentially expressed. Moreover, KEGG pathway enrichment analysis indicated PLA-induced cell membrane damage, energy-metabolism disruption, and central-dogma abnormality in spores. The results provided new insights into the anti- and -AFB mechanisms of PLA.

Citing Articles

biocontrol: antagonism and mycotoxin elimination by lactic acid bacteria.

Krishnan S, Nampoothiri K, Suresh A, Linh N, Balakumaran P, Pocsi I Front Microbiol. 2024; 14:1260166.

PMID: 38235432 PMC: 10791833. DOI: 10.3389/fmicb.2023.1260166.

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