Liposomes Loaded with Vitamin D3 Induce Regulatory Circuits in Human Dendritic Cells

Overview

Journal Front Immunol

Specialty Allergy & Immunology

Date 2023 Jun 26

PMID 37359530

Authors

Noemi Anna Nagy

Fernando Lozano Vigario

Rinske Sparrius

Toni M M van Capel

Ronald van Ree

Sander W Tas

I Jolanda M de Vries

Teunis B H Geijtenbeek

Bram Slutter

Esther C de Jong

Affiliations

Soon will be listed here.

Abstract

Introduction: Nanomedicine provides a promising platform for manipulating dendritic cells (DCs) and the ensuing adaptive immune response. For the induction of regulatory responses, DCs can be targeted with nanoparticles incorporating tolerogenic adjuvants and auto-antigens or allergens.

Methods: Here, we investigated the tolerogenic effect of different liposome formulations loaded with vitamin D3 (VD3). We extensively phenotyped monocyte-derived DCs (moDCs) and skin DCs and assessed DC-induced regulatory CD4+ T cells in coculture.

Results: Liposomal VD3 primed-moDCs induced the development of regulatory CD4+ T cells (Tregs) that inhibited bystander memory T cell proliferation. Induced Tregs were of the FoxP3+ CD127low phenotype, also expressing TIGIT. Additionally, liposome-VD3 primed moDCs inhibited the development of T helper 1 (Th1) and T helper 17 (Th17) cells. Skin injection of VD3 liposomes selectively stimulated the migration of CD14+ skin DCs.

Discussion: These results suggest that nanoparticulate VD3 is a tolerogenic tool for DC-mediated induction of regulatory T cell responses.

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