Flow Cytometry Identification of Cell Compartments in the Murine Brain

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2023 Jun 24

PMID 37355546

Authors

Joel J D Moffet

Zachery Moore

Shannon J Oliver

Tahnee Towers

Misty R Jenkins

Saskia Freytag

James R Whittle

Sarah A Best

Affiliations

Soon will be listed here.

Abstract

Glioma can be modelled in the murine brain through the induction of genetically engineered mouse models or intracranial transplantation. Gliomas (oligodendroglioma and astrocytoma) are thought to arise from neuronal and glial progenitor populations in the brain and are poorly infiltrated by immune cells. An improved understanding of oligodendrocytes, astrocytes, and the immune environment throughout tumor development will enhance the analysis and development of brain cancer models. Here, we describe the isolation and analysis of murine brain cell types using a combination of flow cytometry and quantitative RT-PCR strategies to analyze these individual cell populations in vivo.

Citing Articles

Matrix Selection for the Visualization of Small Molecules and Lipids in Brain Tumors Using Untargeted MALDI-TOF Mass Spectrometry Imaging.

Lu T, Freytag L, Narayana V, Moore Z, Oliver S, Valkovic A Metabolites. 2023; 13(11).

PMID: 37999235 PMC: 10673325. DOI: 10.3390/metabo13111139.

References

Sanai N, Alvarez-Buylla A, Berger M . Neural stem cells and the origin of gliomas. N Engl J Med. 2005; 353(8):811-22. DOI: 10.1056/NEJMra043666. View

Louis D, Perry A, Wesseling P, Brat D, Cree I, Figarella-Branger D . The 2021 WHO Classification of Tumors of the Central Nervous System: a summary. Neuro Oncol. 2021; 23(8):1231-1251. PMC: 8328013. DOI: 10.1093/neuonc/noab106. View

Barthel F, Johnson K, Varn F, Moskalik A, Tanner G, Kocakavuk E . Longitudinal molecular trajectories of diffuse glioma in adults. Nature. 2019; 576(7785):112-120. PMC: 6897368. DOI: 10.1038/s41586-019-1775-1. View

Mweempwa A, Rosenthal M, Dimou J, Drummond K, Whittle J . Perioperative clinical trials for glioma: Raising the bar. J Clin Neurosci. 2021; 89:144-150. DOI: 10.1016/j.jocn.2021.04.026. View

Davis M . Glioblastoma: Overview of Disease and Treatment. Clin J Oncol Nurs. 2016; 20(5 Suppl):S2-8. PMC: 5123811. DOI: 10.1188/16.CJON.S1.2-8. View

Venkatesh H, Morishita W, Geraghty A, Silverbush D, Gillespie S, Arzt M . Electrical and synaptic integration of glioma into neural circuits. Nature. 2019; 573(7775):539-545. PMC: 7038898. DOI: 10.1038/s41586-019-1563-y. View

Venkatesh H, Johung T, Caretti V, Noll A, Tang Y, Nagaraja S . Neuronal Activity Promotes Glioma Growth through Neuroligin-3 Secretion. Cell. 2015; 161(4):803-16. PMC: 4447122. DOI: 10.1016/j.cell.2015.04.012. View

Alcantara Llaguno S, Chen J, Kwon C, Jackson E, Li Y, Burns D . Malignant astrocytomas originate from neural stem/progenitor cells in a somatic tumor suppressor mouse model. Cancer Cell. 2008; 15(1):45-56. PMC: 2650425. DOI: 10.1016/j.ccr.2008.12.006. View

Holland E, Celestino J, Dai C, Schaefer L, Sawaya R, Fuller G . Combined activation of Ras and Akt in neural progenitors induces glioblastoma formation in mice. Nat Genet. 2000; 25(1):55-7. DOI: 10.1038/75596. View

10.

Zheng H, Ying H, Yan H, Kimmelman A, Hiller D, Chen A . p53 and Pten control neural and glioma stem/progenitor cell renewal and differentiation. Nature. 2008; 455(7216):1129-33. PMC: 4051433. DOI: 10.1038/nature07443. View

11.

Wang Z, Sun D, Chen Y, Xie X, Shi Y, Tabar V . Cell Lineage-Based Stratification for Glioblastoma. Cancer Cell. 2020; 38(3):366-379.e8. PMC: 7494533. DOI: 10.1016/j.ccell.2020.06.003. View

12.

Seyfried T, Roy M . Ganglioside distribution in murine neural tumors. Mol Chem Neuropathol. 1992; 17(2):147-67. DOI: 10.1007/BF03159989. View

13.

Pombo Antunes A, Scheyltjens I, Duerinck J, Neyns B, Movahedi K, Van Ginderachter J . Understanding the glioblastoma immune microenvironment as basis for the development of new immunotherapeutic strategies. Elife. 2020; 9. PMC: 7000215. DOI: 10.7554/eLife.52176. View

14.

Gyoneva S, Hosur R, Gosselin D, Zhang B, Ouyang Z, Cotleur A . deficient microglia exhibit a premature aging transcriptome. Life Sci Alliance. 2019; 2(6). PMC: 6892408. DOI: 10.26508/lsa.201900453. View

15.

Swartzlander D, Propson N, Roy E, Saito T, Saido T, Wang B . Concurrent cell type-specific isolation and profiling of mouse brains in inflammation and Alzheimer's disease. JCI Insight. 2018; 3(13). PMC: 6124528. DOI: 10.1172/jci.insight.121109. View

16.

Sim F, McClain C, Schanz S, Protack T, Windrem M, Goldman S . CD140a identifies a population of highly myelinogenic, migration-competent and efficiently engrafting human oligodendrocyte progenitor cells. Nat Biotechnol. 2011; 29(10):934-41. PMC: 3365580. DOI: 10.1038/nbt.1972. View

17.

Best S, de Souza D, Kersbergen A, Policheni A, Dayalan S, Tull D . Synergy between the KEAP1/NRF2 and PI3K Pathways Drives Non-Small-Cell Lung Cancer with an Altered Immune Microenvironment. Cell Metab. 2018; 27(4):935-943.e4. DOI: 10.1016/j.cmet.2018.02.006. View

18.

Inui M, Ishida Y, Kimura A, Kuninaka Y, Mukaida N, Kondo T . Protective roles of CX3CR1-mediated signals in toxin A-induced enteritis through the induction of heme oxygenase-1 expression. J Immunol. 2010; 186(1):423-31. DOI: 10.4049/jimmunol.1000043. View

19.

Herring C, Simmons R, Freytag S, Poppe D, Moffet J, Pflueger J . Human prefrontal cortex gene regulatory dynamics from gestation to adulthood at single-cell resolution. Cell. 2022; 185(23):4428-4447.e28. DOI: 10.1016/j.cell.2022.09.039. View

20.

Zhang S, Rasai A, Wang Y, Xu J, Bannerman P, Erol D . The Stem Cell Factor Sox2 Is a Positive Timer of Oligodendrocyte Development in the Postnatal Murine Spinal Cord. Mol Neurobiol. 2018; 55(12):9001-9015. PMC: 6173662. DOI: 10.1007/s12035-018-1035-7. View