On Target: An Intrapulmonary Transplantation Method for Modelling Lung Tumor Development in Its Native Microenvironment

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2023 Jun 24

PMID 37355535

Authors

Jackson A McDonald

Leanne Scott

Jessica Van Zuylekom

Steven Holloway

Benjamin J Blyth

Kate D Sutherland

Affiliations

Soon will be listed here.

Abstract

The development of in vivo lung cancer models that faithfully mimic the human disease is a crucial research tool for understanding the molecular mechanisms driving tumorigenesis. Subcutaneous transplantation assays are commonly employed, likely due to their amenability to easily monitor tumor growth and the simplistic nature of the technique to deliver tumor cells. Importantly however, subcutaneous tumors grow in a microenvironment that differs from that resident within the lung. To circumvent this limitation, here we describe the development of an intrapulmonary (iPUL) orthotopic transplantation method that enables the delivery of lung cancer cells, with precision, to the left lung lobe of recipient mice. Critically, this allows for the growth of lung cancer cells within their native microenvironment. The coupling of iPUL transplantation with position emission tomography (PET) imaging permits the serial detection of tumors in vivo and serves as a powerful tool to trace lung tumor growth and dissemination over time in mouse disease models.

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