Organized B Cell Sites in Cartilaginous Fishes Reveal the Evolutionary Foundation of Germinal Centers

Overview

Journal Cell Rep

Publisher Cell Press

Specialties Biology
Cell Biology
Molecular Biology

Date 2023 Jun 21

PMID 37342909

Authors

Hanover Matz

Richard S Taylor

Anthony K Redmond

Thomas M Hill

Rose Ruiz Daniels

Mariana Beltran

Neil C Henderson

Daniel J Macqueen

Helen Dooley

Affiliations

Soon will be listed here.

Abstract

The absence of germinal centers (GCs) in cartilaginous fishes lies at odds with data showing that nurse sharks can produce robust antigen-specific responses and affinity mature their B cell repertoires. To investigate this apparent incongruity, we performed RNA sequencing on single nuclei, allowing us to characterize the cell types present in the nurse shark spleen, and RNAscope to provide in situ cellular resolution of key marker gene expression following immunization with R-phycoerythrin (PE). We tracked PE to the splenic follicles where it co-localizes with CXCR5 centrocyte-like B cells and a population of putative T follicular helper (Tfh) cells, surrounded by a peripheral ring of Ki67 AID CXCR4 centroblast-like B cells. Further, we reveal selection of mutations in B cell clones dissected from these follicles. We propose that the B cell sites identified here represent the evolutionary foundation of GCs, dating back to the jawed vertebrate ancestor.

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