Unlocking Chloride Sensing in the Red at Physiological PH with a Fluorescent Rhodopsin-based Host

Overview

Journal Chem Commun (Camb)

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2023 Jun 20

PMID 37337864

Authors

Shelby M Phelps

Jasmine N Tutol

Deeya Advani

Weicheng Peng

Sheel C Dodani

Affiliations

Soon will be listed here.

Abstract

Chloride is a vital ion for all forms of life. Protein-based fluorescent biosensors can enable researchers to visualize chloride in cells but remain underdeveloped. Here, we demonstrate how a single point mutation in an engineered microbial rhodopsin results in ChloRED-1-CFP. This membrane-bound host is a far-red emitting, ratiometric sensor that provides a reversible readout of chloride in live bacteria at physiological pH, setting the stage to investigate the roles of chloride in diverse biological contexts.

Citing Articles

Illuminating anions in biology with genetically encoded fluorescent biosensors.

Cook M, Phelps S, Tutol J, Adams D, Dodani S Curr Opin Chem Biol. 2024; 84:102548.

PMID: 39657518 PMC: 11788029. DOI: 10.1016/j.cbpa.2024.102548.

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