A Plant RNA Virus Inhibits NPR1 Sumoylation and Subverts NPR1-mediated Plant Immunity

Overview

Journal Nat Commun

Specialty Biology

Date 2023 Jun 16

PMID 37328517

Authors

Jiahui Liu

Xiaoyun Wu

Yue Fang

Ye Liu

Esther Oreofe Bello

Yong Li

Ruyi Xiong

Yinzi Li

Zheng Qing Fu

Aiming Wang

Xiaofei Cheng

Affiliations

Soon will be listed here.

Abstract

NONEXPRESSER OF PATHOGENESIS-RELATED GENES 1 (NPR1) is the master regulator of salicylic acid-mediated basal and systemic acquired resistance in plants. Here, we report that NPR1 plays a pivotal role in restricting compatible infection by turnip mosaic virus, a member of the largest plant RNA virus genus Potyvirus, and that such resistance is counteracted by NUCLEAR INCLUSION B (NIb), the viral RNA-dependent RNA polymerase. We demonstrate that NIb binds to the SUMO-interacting motif 3 (SIM3) of NPR1 to prevent SUMO3 interaction and sumoylation, while sumoylation of NIb by SUMO3 is not essential but can intensify the NIb-NPR1 interaction. We discover that the interaction also impedes the phosphorylation of NPR1 at Ser11/Ser15. Moreover, we show that targeting NPR1 SIM3 is a conserved ability of NIb from diverse potyviruses. These data reveal a molecular "arms race" by which potyviruses deploy NIb to suppress NPR1-mediated resistance through disrupting NPR1 sumoylation.

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