Direct Chemical Editing of Gram-positive Bacterial Cell Walls Via an Enzyme-catalyzed Oxidative Coupling Reaction

Overview

Journal Exploration (Beijing)

Specialty Biomedical Engineering

Date 2023 Jun 16

PMID 37325504

Authors

Hao-Ran Jia

Ya-Xuan Zhu

Yi Liu

Yuxin Guo

Sayed Mir Sayed

Xiao-Yu Zhu

Xiaotong Cheng

Fu-Gen Wu

Affiliations

Soon will be listed here.

Abstract

Chemically manipulating bacterial surface structures, a cutting-edge research direction in the biomedical field, predominantly relies on metabolic labeling by now. However, this method may involve daunting precursor synthesis and only labels nascent surface structures. Here, we report a facile and rapid modification strategy based on a tyrosinase-catalyzed oxidative coupling reaction (TyOCR) for bacterial surface engineering. This strategy employs phenol-tagged small molecules and tyrosinase to initiate direct chemical modification of Gram-positive bacterial cell walls with high labeling efficiency, while Gram-negative bacteria are inert to this modification due to the hindrance of an outer membrane. By using the biotin‒avidin system, we further present the selective deposition of various materials, including photosensitizer, magnetic nanoparticle, and horseradish peroxidase, on Gram-positive bacterial surfaces, and realize the purification/isolation/enrichment and naked-eye detection of bacterial strains. This work demonstrates that TyOCR is a promising strategy for engineering live bacterial cells.

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