Genomic Characterization of Three Bacteriophages Infecting Donkey-derived Escherichia Coli

Overview

Journal Virus Genes

Specialties Microbiology
Molecular Biology

Date 2023 Jun 15

PMID 37322310

Authors

Jia-Qi Cui

Wen-Hua Liu

Can Zhang

Ling Zou

Hui-Ying Ren

Affiliations

Soon will be listed here.

Abstract

Bacteriophages are an important source of novel genetic diversity. Sequencing of phage genomes can reveal new proteins with potential uses in phage therapy and help unravel the diversity of biological mechanisms by which phages take over the machinery of the host during infection. To expand the available collection of phage genomes, we have isolated, sequenced, and assembled the genome sequences of three phages that infect three pathogenic Escherichia coli strains: vB_EcoM_DE15, vB_EcoM_DE16, and vB_EcoM_DE17. Morphological characterization and genomic analysis indicated that all three phages were strictly lytic and free from integrases, virulence factors, toxins, and antimicrobial resistance genes. All three phages contained tRNAs, and especially, vB_EcoM_DE17 contained 25 tRNAs. The genomic features of these phages indicate that natural phages are capable of lysing pathogenic E.coli and have great potential in the biocontrol of bacteria.

Citing Articles

Genomic characterization of three bacteriophages infecting donkey-derived Escherichia coli.

Cui J, Liu W, Zhang C, Zou L, Ren H Virus Genes. 2023; 59(5):752-762.

PMID: 37322310 DOI: 10.1007/s11262-023-02008-9.

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