Parasite Detection in Seawater Samples

Overview

Journal Microorganisms

Specialty Microbiology

Date 2023 Jun 15

PMID 37317120

Authors

Irene Moro-Martinez

Maite Vazquez-Luis

Jose Rafael Garcia-March

Patricia Prado

Milena Micic

Gaetano Catanese

Affiliations

Soon will be listed here.

Abstract

In this study, we investigated the presence of the parasite , which is a pathogen for the bivalve , in water samples from different environments. Fifteen mantle samples of infected by were used to characterize the ribosomal unit of this parasite. The obtained sequences were employed to develop a method for eDNA detection of . We collected 56 water samples (from aquaria, open sea and sanctuaries) for testing the methodology. In this work, we developed three different PCRs generating amplicons of different lengths to determine the level of degradation of the DNA, since the status of in water and, therefore, its infectious capacity are unknown. The results showed the ability of the method to detect in sea waters from different areas persistent in the environment but with different degrees of DNA fragmentation. This developed method offers a new tool for preventive analysis for monitoring areas and to better understand the life cycle and the spread of this parasite.

Citing Articles

as effective molecular marker for the detection of natural hybridization between the bivalves and .

Catanese G, Vazquez-Luis M, Giacobbe S, Garcia-March J, Zotou M, Patricia P Ecol Evol. 2024; 14(8):e70227.

PMID: 39193167 PMC: 11347391. DOI: 10.1002/ece3.70227.

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