Genetic Approaches for Neural Circuits Dissection in Non-human Primates

Overview

Journal Neurosci Bull

Specialty Neurology

Date 2023 May 31

PMID 37258795

Authors

Ling Li

Zhen Liu

Affiliations

Soon will be listed here.

Abstract

Genetic tools, which can be used for the morphology study of specific neurons, pathway-selective connectome mapping, neuronal activity monitoring, and manipulation with a spatiotemporal resolution, have been widely applied to the understanding of complex neural circuit formation, interactions, and functions in rodents. Recently, similar genetic approaches have been tried in non-human primates (NHPs) in neuroscience studies for dissecting the neural circuits involved in sophisticated behaviors and clinical brain disorders, although they are still very preliminary. In this review, we introduce the progress made in the development and application of genetic tools for brain studies on NHPs. We also discuss the advantages and limitations of each approach and provide a perspective for using genetic tools to study the neural circuits of NHPs.

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References

Israel M, Morgan M, Tay B, Deuis J . Toxins as tools: Fingerprinting neuronal pharmacology. Neurosci Lett. 2018; 679:4-14. DOI: 10.1016/j.neulet.2018.02.001. View

Mehta P, Kreeger L, Wylie D, Pattadkal J, Lusignan T, Davis M . Functional Access to Neuron Subclasses in Rodent and Primate Forebrain. Cell Rep. 2019; 26(10):2818-2832.e8. PMC: 6509701. DOI: 10.1016/j.celrep.2019.02.011. View

Guan S, Zhang S, Zhang Y, Tang S, Yu C . Plaid Detectors in Macaque V1 Revealed by Two-Photon Calcium Imaging. Curr Biol. 2020; 30(5):934-940.e3. DOI: 10.1016/j.cub.2020.01.005. View

Nurminen L, Merlin S, Bijanzadeh M, Federer F, Angelucci A . Top-down feedback controls spatial summation and response amplitude in primate visual cortex. Nat Commun. 2018; 9(1):2281. PMC: 5995810. DOI: 10.1038/s41467-018-04500-5. View

El-Shamayleh Y, Ni A, Horwitz G . Strategies for targeting primate neural circuits with viral vectors. J Neurophysiol. 2016; 116(1):122-34. PMC: 4961743. DOI: 10.1152/jn.00087.2016. View

Graybuck L, Daigle T, Sedeno-Cortes A, Walker M, Kalmbach B, Lenz G . Enhancer viruses for combinatorial cell-subclass-specific labeling. Neuron. 2021; 109(9):1449-1464.e13. PMC: 8610077. DOI: 10.1016/j.neuron.2021.03.011. View

Chansel-Debordeaux L, Bezard E . Local transgene expression and whole-body transgenesis to model brain diseases in nonhuman primate. Animal Model Exp Med. 2019; 2(1):9-17. PMC: 6431118. DOI: 10.1002/ame2.12055. View

Hordeaux J, Buza E, Jeffrey B, Song C, Jahan T, Yuan Y . MicroRNA-mediated inhibition of transgene expression reduces dorsal root ganglion toxicity by AAV vectors in primates. Sci Transl Med. 2020; 12(569). DOI: 10.1126/scitranslmed.aba9188. View

Ziv Y, Ghosh K . Miniature microscopes for large-scale imaging of neuronal activity in freely behaving rodents. Curr Opin Neurobiol. 2015; 32:141-7. DOI: 10.1016/j.conb.2015.04.001. View

10.

Matsuzaki M, Ebina T . Optical deep-cortex exploration in behaving rhesus macaques. Nat Commun. 2021; 12(1):4656. PMC: 8329299. DOI: 10.1038/s41467-021-24988-8. View

11.

Rickels R, Shilatifard A . Enhancer Logic and Mechanics in Development and Disease. Trends Cell Biol. 2018; 28(8):608-630. DOI: 10.1016/j.tcb.2018.04.003. View

12.

Izpisua Belmonte J, Callaway E, Caddick S, Churchland P, Feng G, Homanics G . Brains, genes, and primates. Neuron. 2015; 86(3):617-31. PMC: 4425847. DOI: 10.1016/j.neuron.2015.03.021. View

13.

Xu Y, Zou P, Cohen A . Voltage imaging with genetically encoded indicators. Curr Opin Chem Biol. 2017; 39():1-10. PMC: 5581692. DOI: 10.1016/j.cbpa.2017.04.005. View

14.

Madisen L, Garner A, Shimaoka D, Chuong A, Klapoetke N, Li L . Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Neuron. 2015; 85(5):942-58. PMC: 4365051. DOI: 10.1016/j.neuron.2015.02.022. View

15.

Keaveney M, Tseng H, Ta T, Gritton H, Man H, Han X . A MicroRNA-Based Gene-Targeting Tool for Virally Labeling Interneurons in the Rodent Cortex. Cell Rep. 2018; 24(2):294-303. PMC: 6095194. DOI: 10.1016/j.celrep.2018.06.049. View

16.

Oguchi M, Sakagami M . Dissecting the Prefrontal Network With Pathway-Selective Manipulation in the Macaque Brain-A Review. Front Neurosci. 2022; 16:917407. PMC: 9168219. DOI: 10.3389/fnins.2022.917407. View

17.

Ju N, Jiang R, Macknik S, Martinez-Conde S, Tang S . Long-term all-optical interrogation of cortical neurons in awake-behaving nonhuman primates. PLoS Biol. 2018; 16(8):e2005839. PMC: 6101413. DOI: 10.1371/journal.pbio.2005839. View

18.

Ju N, Li Y, Liu F, Jiang H, Macknik S, Martinez-Conde S . Spatiotemporal functional organization of excitatory synaptic inputs onto macaque V1 neurons. Nat Commun. 2020; 11(1):697. PMC: 7000673. DOI: 10.1038/s41467-020-14501-y. View

19.

Nagai Y, Kikuchi E, Lerchner W, Inoue K, Ji B, Eldridge M . PET imaging-guided chemogenetic silencing reveals a critical role of primate rostromedial caudate in reward evaluation. Nat Commun. 2016; 7:13605. PMC: 5150653. DOI: 10.1038/ncomms13605. View

20.

Adamantidis A, Zhang F, De Lecea L, Deisseroth K . Optogenetics: opsins and optical interfaces in neuroscience. Cold Spring Harb Protoc. 2014; 2014(8):815-22. DOI: 10.1101/pdb.top083329. View