Retinyl Ester Analysis by Orbitrap Mass Spectrometry

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2023 May 29

PMID 37247055

Authors

Jeroen W A Jansen

Maya W Haaker

Esther A Zaal

J Bernd Helms

Affiliations

Soon will be listed here.

Abstract

Retinoids are light-sensitive molecules that are normally detected by UV absorption techniques. Here we describe the identification and quantification of retinyl ester species by high-resolution mass spectrometry. Retinyl esters are extracted by the method of Bligh and Dyer and subsequently separated by HPLC in runs of 40 min. The retinyl esters are identified and quantified by mass spectrometry analysis. This procedure enables the highly sensitive detection and characterization of retinyl esters in biological samples such as hepatic stellate cells.

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