Fluorescence Microscopy in Adeno-Associated Virus Research

Overview

Journal Viruses

Publisher MDPI

Specialty Microbiology

Date 2023 May 27

PMID 37243260

Authors

Susanne K Golm

Wolfgang Hubner

Kristian M Muller

Affiliations

Soon will be listed here.

Abstract

Research on adeno-associated virus (AAV) and its recombinant vectors as well as on fluorescence microscopy imaging is rapidly progressing driven by clinical applications and new technologies, respectively. The topics converge, since high and super-resolution microscopes facilitate the study of spatial and temporal aspects of cellular virus biology. Labeling methods also evolve and diversify. We review these interdisciplinary developments and provide information on the technologies used and the biological knowledge gained. The emphasis lies on the visualization of AAV proteins by chemical fluorophores, protein fusions and antibodies as well as on methods for the detection of adeno-associated viral DNA. We add a short overview of fluorescent microscope techniques and their advantages and challenges in detecting AAV.

Citing Articles

Exploring HIV-1 Maturation: A New Frontier in Antiviral Development.

McGraw A, Hillmer G, Medehincu S, Hikichi Y, Gagliardi S, Narayan K Viruses. 2024; 16(9).

PMID: 39339899 PMC: 11437483. DOI: 10.3390/v16091423.

A novel approach to quantitate biodistribution and transduction of adeno-associated virus gene therapy using radiolabeled AAV vectors in mice.

Wang H, Li R, Sadekar S, Kamath A, Shen B Mol Ther Methods Clin Dev. 2024; 32(3):101326.

PMID: 39286334 PMC: 11404148. DOI: 10.1016/j.omtm.2024.101326.

Open-source and low-cost miniature microscope for on-site fluorescence detection.

Kawai M, Oda H, Mimura H, Osaki T, Takeuchi S HardwareX. 2024; 19:e00545.

PMID: 39006472 PMC: 11239704. DOI: 10.1016/j.ohx.2024.e00545.

References

Lambert T . FPbase: a community-editable fluorescent protein database. Nat Methods. 2019; 16(4):277-278. DOI: 10.1038/s41592-019-0352-8. View

Slanina H, Weger S, Stow N, Kuhrs A, Heilbronn R . Role of the herpes simplex virus helicase-primase complex during adeno-associated virus DNA replication. J Virol. 2006; 80(11):5241-50. PMC: 1472166. DOI: 10.1128/JVI.02718-05. View

Kishi J, Schaus T, Gopalkrishnan N, Xuan F, Yin P . Programmable autonomous synthesis of single-stranded DNA. Nat Chem. 2018; 10(2):155-164. PMC: 5784857. DOI: 10.1038/nchem.2872. View

Fraefel C, Bittermann A, Bueler H, Heid I, Bachi T, Ackermann M . Spatial and temporal organization of adeno-associated virus DNA replication in live cells. J Virol. 2003; 78(1):389-98. PMC: 303420. DOI: 10.1128/jvi.78.1.389-398.2004. View

Meyer N, Chapman M . Adeno-associated virus (AAV) cell entry: structural insights. Trends Microbiol. 2021; 30(5):432-451. PMC: 11225776. DOI: 10.1016/j.tim.2021.09.005. View

ATCHISON R, CASTO B, HAMMON W . ADENOVIRUS-ASSOCIATED DEFECTIVE VIRUS PARTICLES. Science. 1965; 149(3685):754-6. DOI: 10.1126/science.149.3685.754. View

Cui M, Lu Y, Tang C, Zhang R, Wang J, Si Y . A Generic Method for Fast and Sensitive Detection of Adeno-Associated Viruses Using Modified AAV Receptor Recombinant Proteins. Molecules. 2019; 24(21). PMC: 6864843. DOI: 10.3390/molecules24213973. View

Maurer A, Pacouret S, Cepeda Diaz A, Blake J, Andres-Mateos E, Vandenberghe L . The Assembly-Activating Protein Promotes Stability and Interactions between AAV's Viral Proteins to Nucleate Capsid Assembly. Cell Rep. 2018; 23(6):1817-1830. PMC: 5983388. DOI: 10.1016/j.celrep.2018.04.026. View

Le D, Radukic M, Teschner K, Becker L, Muller K . Synthesis and Concomitant Assembly of Adeno-Associated Virus-like Particles in . ACS Synth Biol. 2022; 11(11):3601-3607. DOI: 10.1021/acssynbio.2c00451. View

10.

Wistuba A, Weger S, Kern A, Kleinschmidt J . Intermediates of adeno-associated virus type 2 assembly: identification of soluble complexes containing Rep and Cap proteins. J Virol. 1995; 69(9):5311-9. PMC: 189369. DOI: 10.1128/JVI.69.9.5311-5319.1995. View

11.

Buning H, Perabo L, Coutelle O, Quadt-Humme S, Hallek M . Recent developments in adeno-associated virus vector technology. J Gene Med. 2008; 10(7):717-33. DOI: 10.1002/jgm.1205. View

12.

Earley L, Kawano Y, Adachi K, Sun X, Dai M, Nakai H . Identification and characterization of nuclear and nucleolar localization signals in the adeno-associated virus serotype 2 assembly-activating protein. J Virol. 2015; 89(6):3038-48. PMC: 4337552. DOI: 10.1128/JVI.03125-14. View

13.

Wistuba A, Kern A, Weger S, Grimm D, Kleinschmidt J . Subcellular compartmentalization of adeno-associated virus type 2 assembly. J Virol. 1997; 71(2):1341-52. PMC: 191189. DOI: 10.1128/JVI.71.2.1341-1352.1997. View

14.

Le D, Radukic M, Muller K . Adeno-associated virus capsid protein expression in Escherichia coli and chemically defined capsid assembly. Sci Rep. 2019; 9(1):18631. PMC: 6901487. DOI: 10.1038/s41598-019-54928-y. View

15.

Sakin V, Hanne J, Dunder J, Anders-Osswein M, Laketa V, Nikic I . A Versatile Tool for Live-Cell Imaging and Super-Resolution Nanoscopy Studies of HIV-1 Env Distribution and Mobility. Cell Chem Biol. 2017; 24(5):635-645.e5. DOI: 10.1016/j.chembiol.2017.04.007. View

16.

Popa-Wagner R, Porwal M, Kann M, Reuss M, Weimer M, Florin L . Impact of VP1-specific protein sequence motifs on adeno-associated virus type 2 intracellular trafficking and nuclear entry. J Virol. 2012; 86(17):9163-74. PMC: 3416132. DOI: 10.1128/JVI.00282-12. View

17.

Chang L, Shi Y, Shenk T . Adeno-associated virus P5 promoter contains an adenovirus E1A-inducible element and a binding site for the major late transcription factor. J Virol. 1989; 63(8):3479-88. PMC: 250925. DOI: 10.1128/JVI.63.8.3479-3488.1989. View

18.

Penzes J, Chipman P, Bhattacharya N, Zeher A, Huang R, McKenna R . Adeno-associated Virus 9 Structural Rearrangements Induced by Endosomal Trafficking pH and Glycan Attachment. J Virol. 2021; 95(19):e0084321. PMC: 8428384. DOI: 10.1128/JVI.00843-21. View

19.

Gurda B, Raupp C, Popa-Wagner R, Naumer M, Olson N, Ng R . Mapping a neutralizing epitope onto the capsid of adeno-associated virus serotype 8. J Virol. 2012; 86(15):7739-51. PMC: 3421660. DOI: 10.1128/JVI.00218-12. View

20.

Tokunaga M, Imamoto N, Sakata-Sogawa K . Highly inclined thin illumination enables clear single-molecule imaging in cells. Nat Methods. 2008; 5(2):159-61. DOI: 10.1038/nmeth1171. View