Comparative Analysis of Bone Ingrowth in 3D-Printed Titanium Lattice Structures with Different Patterns

Overview

Journal Materials (Basel)

Publisher MDPI

Date 2023 May 27

PMID 37241487

Authors

Agnes Eva Kovacs

Zoltan Csernatony

Lorand Csamer

Gabor Mehes

Daniel Szabo

Mihaly Veres

Mihaly Braun

Balazs Harangi

Norbert Serban

Lei Zhang

Gyorgy Falk

Hajnalka Soosne Horvath

Sandor Mano

Affiliations

Soon will be listed here.

Abstract

In this study, metal 3D printing technology was used to create lattice-shaped test specimens of orthopedic implants to determine the effect of different lattice shapes on bone ingrowth. Six different lattice shapes were used: gyroid, cube, cylinder, tetrahedron, double pyramid, and Voronoi. The lattice-structured implants were produced from Ti6Al4V alloy using direct metal laser sintering 3D printing technology with an EOS M290 printer. The implants were implanted into the femoral condyles of sheep, and the animals were euthanized 8 and 12 weeks after surgery. To determine the degree of bone ingrowth for different lattice-shaped implants, mechanical, histological, and image processing tests on ground samples and optical microscopic images were performed. In the mechanical test, the force required to compress the different lattice-shaped implants and the force required for a solid implant were compared, and significant differences were found in several instances. Statistically evaluating the results of our image processing algorithm, it was found that the digitally segmented areas clearly consisted of ingrown bone tissue; this finding is also supported by the results of classical histological processing. Our main goal was realized, so the bone ingrowth efficiencies of the six lattice shapes were ranked. It was found that the gyroid, double pyramid, and cube-shaped lattice implants had the highest degree of bone tissue growth per unit time. This ranking of the three lattice shapes remained the same at both 8 and 12 weeks after euthanasia. In accordance with the study, as a side project, a new image processing algorithm was developed that proved suitable for determining the degree of bone ingrowth in lattice implants from optical microscopic images. Along with the cube lattice shape, whose high bone ingrowth values have been previously reported in many studies, it was found that the gyroid and double pyramid lattice shapes produced similarly good results.

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References

Taniguchi N, Fujibayashi S, Takemoto M, Sasaki K, Otsuki B, Nakamura T . Effect of pore size on bone ingrowth into porous titanium implants fabricated by additive manufacturing: An in vivo experiment. Mater Sci Eng C Mater Biol Appl. 2015; 59:690-701. DOI: 10.1016/j.msec.2015.10.069. View

Torres Y, Lascano S, Bris J, Pavon J, Rodriguez J . Development of porous titanium for biomedical applications: A comparison between loose sintering and space-holder techniques. Mater Sci Eng C Mater Biol Appl. 2014; 37:148-55. DOI: 10.1016/j.msec.2013.11.036. View

Derby B . Printing and prototyping of tissues and scaffolds. Science. 2012; 338(6109):921-6. DOI: 10.1126/science.1226340. View

Chang B, Song W, Han T, Yan J, Li F, Zhao L . Influence of pore size of porous titanium fabricated by vacuum diffusion bonding of titanium meshes on cell penetration and bone ingrowth. Acta Biomater. 2016; 33:311-21. DOI: 10.1016/j.actbio.2016.01.022. View

Ryan G, Pandit A, Apatsidis D . Fabrication methods of porous metals for use in orthopaedic applications. Biomaterials. 2006; 27(13):2651-70. DOI: 10.1016/j.biomaterials.2005.12.002. View

Pobloth A, Mersiowsky M, Kliemt L, Schell H, Dienelt A, Pfitzner B . Bioactive coating of zirconia toughened alumina ceramic implants improves cancellous osseointegration. Sci Rep. 2019; 9(1):16692. PMC: 6853946. DOI: 10.1038/s41598-019-53094-5. View

Parthasarathy J, Starly B, Raman S, Christensen A . Mechanical evaluation of porous titanium (Ti6Al4V) structures with electron beam melting (EBM). J Mech Behav Biomed Mater. 2010; 3(3):249-59. DOI: 10.1016/j.jmbbm.2009.10.006. View

Lim H, Ryu M, Woo S, Song I, Choi Y, Lee U . Bone Conduction Capacity of Highly Porous 3D-Printed Titanium Scaffolds Based on Different Pore Designs. Materials (Basel). 2021; 14(14). PMC: 8303426. DOI: 10.3390/ma14143892. View

Wang C, Sun B, Zhang Y, Wang C, Yang G . Design of a Novel Trabecular Acetabular Cup and Selective Laser Melting Fabrication. Materials (Basel). 2022; 15(17). PMC: 9457748. DOI: 10.3390/ma15176142. View

10.

Vafaeefar M, Moerman K, Kavousi M, Vaughan T . A morphological, topological and mechanical investigation of gyroid, spinodoid and dual-lattice algorithms as structural models of trabecular bone. J Mech Behav Biomed Mater. 2022; 138:105584. DOI: 10.1016/j.jmbbm.2022.105584. View

11.

Chen Z, Yan X, Yin S, Liu L, Liu X, Zhao G . Influence of the pore size and porosity of selective laser melted Ti6Al4V ELI porous scaffold on cell proliferation, osteogenesis and bone ingrowth. Mater Sci Eng C Mater Biol Appl. 2019; 106:110289. DOI: 10.1016/j.msec.2019.110289. View

12.

Deng F, Liu L, Li Z, Liu J . 3D printed Ti6Al4V bone scaffolds with different pore structure effects on bone ingrowth. J Biol Eng. 2021; 15(1):4. PMC: 7818551. DOI: 10.1186/s13036-021-00255-8. View

13.

Park K, Min K, Roh Y . Design Optimization of Lattice Structures under Compression: Study of Unit Cell Types and Cell Arrangements. Materials (Basel). 2022; 15(1). PMC: 8746100. DOI: 10.3390/ma15010097. View

14.

Luo C, Wang C, Wu X, Xie X, Wang C, Zhao C . Influence of porous tantalum scaffold pore size on osteogenesis and osteointegration: A comprehensive study based on 3D-printing technology. Mater Sci Eng C Mater Biol Appl. 2021; 129:112382. DOI: 10.1016/j.msec.2021.112382. View

15.

Kelly C, Wang T, Crowley J, Wills D, Pelletier M, Westrick E . High-strength, porous additively manufactured implants with optimized mechanical osseointegration. Biomaterials. 2021; 279:121206. DOI: 10.1016/j.biomaterials.2021.121206. View

16.

Boyan B, Schwartz Z, Lohmann C, Sylvia V, Cochran D, Dean D . Pretreatment of bone with osteoclasts affects phenotypic expression of osteoblast-like cells. J Orthop Res. 2003; 21(4):638-47. DOI: 10.1016/S0736-0266(02)00261-9. View

17.

Pearce A, Richards R, Milz S, Schneider E, Pearce S . Animal models for implant biomaterial research in bone: a review. Eur Cell Mater. 2007; 13:1-10. DOI: 10.22203/ecm.v013a01. View

18.

Li F, Li J, Xu G, Liu G, Kou H, Zhou L . Fabrication, pore structure and compressive behavior of anisotropic porous titanium for human trabecular bone implant applications. J Mech Behav Biomed Mater. 2015; 46:104-14. DOI: 10.1016/j.jmbbm.2015.02.023. View

19.

Kladovasilakis N, Tsongas K, Karalekas D, Tzetzis D . Architected Materials for Additive Manufacturing: A Comprehensive Review. Materials (Basel). 2022; 15(17). PMC: 9456607. DOI: 10.3390/ma15175919. View

20.

Karageorgiou V, Kaplan D . Porosity of 3D biomaterial scaffolds and osteogenesis. Biomaterials. 2005; 26(27):5474-91. DOI: 10.1016/j.biomaterials.2005.02.002. View