Gonadotropins and Sex Steroid Hormones in Captive-Reared Small Yellow Croaker () and Their Role in Female Reproductive Dysfunction

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2023 May 27

PMID 37240265

Authors

Kang Hee Kho

Zahid Parvez Sukhan

Seok-Woo Yang

Nam-Yong Hwang

Won-Kyo Lee

Affiliations

Soon will be listed here.

Abstract

The seed production of small yellow croaker (SYC) is constrained by reproductive dysfunction in captive-reared females. Reproductive dysfunction is closely linked to endocrine reproductive mechanisms. To better understand the reproductive dysfunction in captive broodstock, functional characterization of gonadotropins (GtHs: follicle stimulating hormone β subunit, luteinizing hormone β subunit, and glycoprotein α subunit, ) and sex steroids (17β-estradiol, E2; testosterone, T; progesterone; P) was performed using qRT-PCR, ELISA, in vivo, and in-vitro assay. The pituitary GtHs and gonadal steroids levels were significantly higher in ripen fish of both sexes. However, changes in and E2 levels in females were not significant in the developing and ripen stages. Furthermore, GtHs and steroids levels were lower in females compared to males throughout the reproductive cycle. In vivo administration of gonadotropin releasing hormone analogue (GnRHa) significantly increased the expression of GtHs in both dose- and time-related manners. The lower and higher doses of GnRHa led to successful spawning in male and female SYC, respectively. Sex steroids in vitro significantly inhibited the expression of in female SYC. Overall, GtHs were shown to play a vital role in final gonadal maturation, while steroids promoted negative feedback in the regulation of pituitary GtHs. Lower levels of GtHs and steroids might be key components in the reproductive dysfunction of captive-reared female SYC.

References

Zohar Y, Munoz-Cueto J, Elizur A, Kah O . Neuroendocrinology of reproduction in teleost fish. Gen Comp Endocrinol. 2009; 165(3):438-55. DOI: 10.1016/j.ygcen.2009.04.017. View

Guzman J, Cal R, Garcia-Lopez A, Chereguini O, Kight K, Olmedo M . Effects of in vivo treatment with the dopamine antagonist pimozide and gonadotropin-releasing hormone agonist (GnRHa) on the reproductive axis of Senegalese sole (Solea senegalensis). Comp Biochem Physiol A Mol Integr Physiol. 2010; 158(2):235-45. DOI: 10.1016/j.cbpa.2010.11.016. View

Shahjahan M, Hamabata T, Motohashi E, Doi H, Ando H . Differential expression of three types of gonadotropin-releasing hormone genes during the spawning season in grass puffer, Takifugu niphobles. Gen Comp Endocrinol. 2010; 167(1):153-63. DOI: 10.1016/j.ygcen.2010.01.018. View

Khakoo Z, Bhatia A, Gedamu L, Habibi H . Functional specificity for salmon gonadotropin-releasing hormone (GnRH) and chicken GnRH-II coupled to the gonadotropin release and subunit messenger ribonucleic acid level in the goldfish pituitary. Endocrinology. 1994; 134(2):838-47. DOI: 10.1210/endo.134.2.7507838. View

Wong T, Zohar Y . Novel expression of gonadotropin subunit genes in oocytes of the gilthead seabream (Sparus aurata). Endocrinology. 2004; 145(11):5210-20. DOI: 10.1210/en.2004-0558. View

So W, Kwok H, Ge W . Zebrafish gonadotropins and their receptors: II. Cloning and characterization of zebrafish follicle-stimulating hormone and luteinizing hormone subunits--their spatial-temporal expression patterns and receptor specificity. Biol Reprod. 2005; 72(6):1382-96. DOI: 10.1095/biolreprod.104.038216. View

Chen H, Cui X, Wang Y, Li Z, Tian C, Jiang D . Identification, functional characterization, and estrogen regulation on gonadotropin-releasing hormone in the spotted scat, Scatophagus argus. Fish Physiol Biochem. 2020; 46(5):1743-1757. DOI: 10.1007/s10695-020-00825-5. View

Sukhan Z, Cho Y, Hossen S, Yang S, Hwang N, Lee W . Functional Characterization of Three GnRH Isoforms in Small Yellow Croaker Maintained in Captivity: Special Emphasis on Reproductive Dysfunction. Biology (Basel). 2022; 11(8). PMC: 9404844. DOI: 10.3390/biology11081200. View

Gen K, Okuzawa K, Senthilkumaran B, Tanaka H, Moriyama S, Kagawa H . Unique expression of gonadotropin-I and -II subunit genes in male and female red seabream (Pagrus major) during sexual maturation. Biol Reprod. 2000; 63(1):308-19. DOI: 10.1095/biolreprod63.1.308. View

10.

Omony J, Biran J, Kahwa D, Aizen J, Golan M, Nyatia E . Cloning of gonadotropin Gph-alpha, FSH-beta and LH-beta subunits and seasonal profiles of steroid hormones in wild-caught Nile perch, Lates niloticus. Gen Comp Endocrinol. 2022; 323-324:114035. DOI: 10.1016/j.ygcen.2022.114035. View

11.

Shi B, Liu X, Xu Y, Sun Z, Chen S, Zang K . Molecular and transcriptional characterization of GTHs and mPRα during ovarian maturation in rock bream Oplegnathus fasciatus. J Exp Zool A Ecol Genet Physiol. 2015; 323(7):430-44. DOI: 10.1002/jez.1939. View

12.

Pandolfi M, Pozzi A, Canepa M, Vissio P, Shimizu A, Maggese M . Presence of beta-follicle-stimulating hormone and beta-luteinizing hormone transcripts in the brain of Cichlasoma dimerus (Perciformes: Cichlidae): effect of brain-derived gonadotropins on pituitary hormone release. Neuroendocrinology. 2008; 89(1):27-37. DOI: 10.1159/000152833. View

13.

Hassin S, Gothilf Y, Blaise O, Zohar Y . Gonadotropin-I and -II subunit gene expression of male striped bass (Morone saxatilis) after gonadotropin-releasing hormone analogue injection: quantitation using an optimized ribonuclease protection assay. Biol Reprod. 1998; 58(5):1233-40. DOI: 10.1095/biolreprod58.5.1233. View

14.

Nagahama Y, Yoshikuni M, Yamashita M, Tokumoto T, Katsu Y . Regulation of oocyte growth and maturation in fish. Curr Top Dev Biol. 1995; 30:103-45. DOI: 10.1016/s0070-2153(08)60565-7. View

15.

Mylonas C, Fostier A, Zanuy S . Broodstock management and hormonal manipulations of fish reproduction. Gen Comp Endocrinol. 2009; 165(3):516-34. DOI: 10.1016/j.ygcen.2009.03.007. View

16.

Yaron Z, Gur G, Melamed P, Rosenfeld H, Elizur A, Levavi-Sivan B . Regulation of fish gonadotropins. Int Rev Cytol. 2003; 225:131-85. DOI: 10.1016/s0074-7696(05)25004-0. View

17.

Crim L, Evans D . Seasonal levels of pituitary and plasma gonadotropin in male and female Atlantic salmon parr. Can J Zool. 1978; 56(7):1550-5. DOI: 10.1139/z78-216. View

18.

Shi B, Liu X, Xu Y, Wang S . Molecular characterization of three gonadotropin subunits and their expression patterns during ovarian maturation in Cynoglossus semilaevis. Int J Mol Sci. 2015; 16(2):2767-93. PMC: 4346864. DOI: 10.3390/ijms16022767. View

19.

Moreira R, Honji R, Melo R, de Moraes Narcizo A, Amaral J, de Carvalho Araujo R . The involvement of gonadotropins and gonadal steroids in the ovulatory dysfunction of the potamodromous Salminus hilarii (Teleostei: Characidae) in captivity. Fish Physiol Biochem. 2015; 41(6):1435-47. DOI: 10.1007/s10695-015-0097-y. View

20.

Guzman J, Luckenbach J, Swanson P . Molecular characterization and quantification of sablefish (Anoplopoma fimbria) gonadotropins and their receptors: reproductive dysfunction in female captive broodstock. Gen Comp Endocrinol. 2013; 193:37-47. DOI: 10.1016/j.ygcen.2013.07.007. View