Molecular Characterization of Three Gonadotropin Subunits and Their Expression Patterns During Ovarian Maturation in Cynoglossus Semilaevis

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2015 Jan 31

PMID 25633101

Citations 6

Authors

Bao Shi

Xuezhou Liu

Yongjiang Xu

Shanshan Wang

Affiliations

Soon will be listed here.

Abstract

The endocrine regulation of reproduction in a multiple spawning flatfish with an ovary of asynchronous development remains largely unknown. The objectives of this study were to monitor changes in mRNA expression patterns of three gonadotropin hormone (GTH) subunits (FSHβ, LHβ and CGα) and plasma GTH levels during ovarian maturation of half-smooth tongue sole Cynoglossus semilaevis. Cloning and sequence analysis revealed that the cDNAs of FSHβ, LHβ and CGα were 541, 670 and 685 bp in length, and encode for peptides of 130, 158 and 127 amino acids, respectively. The number of cysteine residues and potential N-linked glycosylation sites of the flatfish GTHs were conserved among teleosts. However, the primary structure of GTHs in Pleuronectiformes appeared to be highly divergent. The FSHβ transcriptional level in the pituitary remained high during the vitellogenic stage while plasma levels of FSH peaked and oocyte development was stimulated. The LHβ expression in the pituitary and ovary reached the maximum level during oocyte maturation stages when the plasma levels of LH peaked. The brain GTHs were expressed at the different ovarian stages. These results suggested that FSH and LH may simultaneously regulate ovarian development and maturation through the brain-pituitary-ovary axis endocrine system in tongue sole.

Citing Articles

Gonadotropins and Sex Steroid Hormones in Captive-Reared Small Yellow Croaker () and Their Role in Female Reproductive Dysfunction.

Kho K, Sukhan Z, Yang S, Hwang N, Lee W Int J Mol Sci. 2023; 24(10).

PMID: 37240265 PMC: 10219107. DOI: 10.3390/ijms24108919.

New evidence for SPX2 in regulating the brain-pituitary reproductive axis of half-smooth tongue sole ().

Wang B, Wang K, Tian Z, Cui A, Liu X, Jin Z Front Endocrinol (Lausanne). 2022; 13:984797.

PMID: 35979437 PMC: 9376245. DOI: 10.3389/fendo.2022.984797.

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Dong Y, Lyu L, Wen H, Shi B Front Genet. 2021; 12:802953.

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Comprehensive CircRNA Profiling and Selection of Key CircRNAs Reveal the Potential Regulatory Roles of CircRNAs throughout Ovarian Development and Maturation in .

Li J, Shi B, Wang C, Shao C, Liu X, Zhang D Biology (Basel). 2021; 10(9).

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Characterization of kiss2 and kissr2 genes and the regulation of kisspeptin on the HPG axis in Cynoglossus semilaevis.

Song H, Wang M, Wang Z, Liu J, Qi J, Zhang Q Fish Physiol Biochem. 2017; 43(3):731-753.

PMID: 28120214 DOI: 10.1007/s10695-016-0328-x.

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