Tolerability and Tropism of Recombinant Adeno-associated Virus Vectors in the African Green Monkey (Chlorocebus Sabaeus) Anterior Chamber

Overview

Journal Gene Ther

Date 2023 May 23

PMID 37221271

Authors

Kristina J Chern

Kimicia Z Issac

Zendorf D Gumbs

Merissa E OConnor

Matthew S Lawrence

Daniel M Lipinski

Affiliations

Soon will be listed here.

Abstract

While many studies have investigated the use of recombinant adeno-associated vectors (rAAV) in the posterior chamber for treatment of inherited retinal diseases, fewer studies have looked at rAAV's ability to transduce cells within the anterior chamber. This study focuses on evaluating the tropism and tolerability of three rAAV serotypes-rAAV2/6, rAAV2/9, and rAAV2/2[MAX]-expressing a green fluorescent protein (GFP) reporter following intracameral injection in the non-human primate (NHP) African green monkey (Chlorocebus sabaeus) model. Injection of high dose (1 × 10 vg/eye) rAAV vector resulted in transient inflammation characterized by aqueous flare and cellular infiltrate that resolved without intervention in all serotypes. Post-mortem histology revealed widespread expression of GFP in cells of the trabecular meshwork and iris in high dose rAAV2/6, rAAV2/9, and particularly rAAV2/2[MAX] eyes, indicating that rAAV vectors of these serotypes have broad tropism for cells of the anterior chamber and may facilitate the treatment of blinding disorders, such as glaucoma.

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