The Synergistic Effect of IBRC-M10790 and Vitamin D3 on -induced Inflammation

Background: Owing to the emergence and spread of multidrug resistance mechanisms in , achieving a successful eradication has become exceedingly difficult. Thus, this study for the first time determines the effect of a combination of vitamin D3 and probiotic on the pathogenesis and treatment of .

Methods: We established an experimental system using AGS human gastric carcinoma cells and explored the synergistic effect of IBRC-M10790 and vitamin D3 on . Live and pasteurized , -derived membrane vesicles (MVs), and cell-free supernatant (CFS), as well as their combination with vitamin D3 were used during this study. We assessed the anti-inflammatory and anti-oxidative effects of these combinations using RT-qPCR and ELISA, respectively. We further performed an adhesion assay to evaluate the influence of and vitamin D3 on the adherence rate of to AGS cells.

Results: Our results demonstrated that and vitamin D3 possess anti-inflammatory and anti-oxidative effects against infection in AGS cells. The combination of vitamin D3 with the probiotic strain (particularly live and its CFS) can more efficiently reduce the expression of pro-inflammatory cytokines IL-6, IL-8, IFN-γ, and TNF-α in the AGS cells. Moreover, vitamin D3 and exhibited an additive impact preserving the integrity of the epithelial barrier by increasing the expression of the tight junction protein ZO-1. Furthermore, this combination can potentially reduce adherence to AGS cells.

Conclusions: This study indicates the advantage of combining vitamin D3 and probiotic to attenuate -induced inflammation and oxidative stress. Consequently, probiotic and vitamin D3 co-supplementation can be considered as a novel therapeutic approach to manage and prevent infection.